香蕉根际及非根际土壤细菌群落特征及共性规律研究  

Study on the characteristics and common rule of bacteria community in banana rhizosphere and non-rhizosphere soil

作　　者：黄穗萍[1] 金德才[2] 李其利[1] 韦绍龙[3] 唐利华[1] 黄素梅[3] 郭堂勋[1] 罗述名 莫贱友[1] HUANG Sui-ping;JIN De-cai;LI Qi-li;WEI Shao-long;TANG Li-hua;HUANG Su-mei;GUO Tang-xun;LUO Shu-ming;MO Jian-you(Institute of Plant Protection,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning,Guangxi 530007,China;Key Laboratory of Environmental Biotechnology,Research Center for Eco-environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Institute of Biotechnology Research,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China;Institute of Flowers Research,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China)

机构地区：[1]广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室,广西南宁530007 [2]中国科学院生态环境研究中心环境生物技术重点实验室,北京100085 [3]广西农业科学院生物技术研究所,广西南宁530007 [4]广西农业科学院花卉研究所,广西南宁530007

出　　处：《南方农业学报》2022年第5期1253-1262,共10页Journal of Southern Agriculture

基　　金：广西科技重大专项(桂科AA18118028-6);广西自然科学基金青年科学基金项目(2018GXNSFBA281077);广西自然科学基金面上项目(2020GXNSFAA259064);广西农业科学院基本科研业务专项(桂农科2022YM06)。

摘　　要：【目的】探究广西不同地区香蕉种植园的土壤细菌多样性特征及差异,为揭示不同地区香蕉根际细菌的共性特征及制定调控策略提供理论基础。【方法】采集广西3个香蕉主产区(龙州县、隆安县和浦北县)香蕉种植园的根际和非根际土壤样品共48份,利用扩增子测序技术对48份土壤样品的细菌丰度与多样性进行测定,同时对48份土壤样品的pH及全氮(TN)、全磷(TP)、总有机质(SOM)、速效磷(AP)、硝态氮(NN)、铵态氮(AN)、速效钾(AK)含量等土壤理化性质进行检测,利用T检验算法比较香蕉根际与非根际的土壤环境因子、土壤细菌多样性指数以及土壤细菌物种丰度间的差异,采用ADONIS、ANOSIM和MRPP分析组间的细菌群落结构差异水平,采用Mantel test分析确定影响细菌群落结构的重要环境因子。【结果】3个地区香蕉种植园根际土壤细菌群落的丰度与多样性均较非根际的丰富,同时香蕉根际与非根际土壤细菌群落结构均存在极显著差异(P<0.01),并共同受土壤pH的驱动;香蕉种植园的土壤细菌以变形菌门(Proteobacteria)和酸杆菌门(Acidobacteria)为主要门类,且均能招募芽单胞菌属(Gemmatimonas)、消化螺旋菌属(Nitrospira)和鞘氨醇单胞菌属(Sphingomonas)等优势菌群,而过滤酸杆菌属(Acidobacterium)Gp2、Gp3、Gp6和亚硝化球菌属(Nitrososphaera);FAPROTAX预测结果表明,根际土壤能富集化能异养(Chemoheterotrophy)和好氧化能异养(Aerobic-chemoheterotrophy)等相关功能的菌群。【结论】香蕉根际能富集有益菌群并招募有益的功能细菌,土壤pH对3个地区香蕉种植园的细菌群落均起着重要的调节作用。【Objective】To explore the characteristics and differences of soil bacteria diversity of banana plantations in different regions of Guangxi,so as to provide a theoretical basis for revealing the common characteristics of banana soil microorganisms and how to regulate it in different regions.【Method】Forty-eight samples of banana rhizosphere and nonrhizosphere soil were collected from three regions of Guangxi(Longzhou County,Longan County and Pubei County).The bacteria abundance and diversity of 48 soil samples were determined by the high-throughput sequencing technology.Soil pH,total nitrogen(TN),total phosphorus(TP),total organic matter(SOM),available phosphorus(AP),nitrate nitrogen(NN),ammonium nitrogen(AN),and available potassium(AK)contents were also determined.The T-test algorithm was used to compare the differences between the two groups of soil environmental factors,diversity index and species abundance;ADONIS,ANOSIM and MRPP were used to analyze the difference level of bacteria community structure between the two groups;Mantel test was used to analyze and determine the important environmental factors affecting the community structure.【Result】The results showed that the abundance and diversity of bacteria community of rhizosphere soil was higher than that of non-rhizosphere soil in all the three banana plantations.Meanwhile,the significant difference of bacteria community structure was also observed between banana rhizosphere and non-rhizosphere soil(P<0.01),and this was caused by the pH of the soil.The dominant bacteria communities were Proteobacteria and Acidobacteria at phylum level,and rhizosphere soil of all the three banana plantations could recruit beneficial bacteria such as Gemmatimonas,Nitrospira,Sphingomonas,etc.and filter out Acidobacterium Gp2,Gp3,Gp6,Nitrososphaera.FAPROTAX prediction showed that rhizosphere soil could enrich bacteria communities related to chemoheterotrophy and aerobic-chemoheterotrophy.【Conclusion】The banana rhizosphere can enrich beneficial bacteria communiti

关 键 词：香蕉 根际土壤 细菌多样性 群落结构 pH 广西 

分 类 号：S668.1[农业科学—果树学]