深绿木霉T2菌株发酵液蛋白提取物TraT2A抑菌条件优化及其对百合叶斑病的田间防治效果  

Optimization of inhibition conditions of Trichoderma atroviride T2 fermentation liquid protein extract TraT2A and its field control efficacy for lily leaf spot disease

作　　者：陈应娥 梁巧兰 魏列新 同发宇 王冬 CHEN Ying’e;LIANG Qiaolan;WEI Liexin;TONG Fayu;WANG Dong(College of Plant Protection,Gansu Agricultural University,Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,甘肃省农作物病虫害生物防治工程实验室,兰州730070

出　　处：《农药学学报》2022年第4期782-788,共7页Chinese Journal of Pesticide Science

基　　金：甘肃省农牧厅项目(GNSW-2016-25);兰州市科技局项目(2018-4-78);甘肃省科技重大专项(18ZD2NA010);甘肃农业大学学科建设基金(GAU-XKJS-2018-155).

摘　　要：为了提高深绿木霉Trichoderma atroviride T2菌株发酵液蛋白提取物TraT2A的抑菌活性和光稳定性,采用菌丝生长速率法,通过对紫外保护剂(抗坏血酸、腐殖酸)和辅助剂(IE-08、SP-4821A和SP-4821B)的筛选,优化了TraT2A对百合叶斑病菌Alternaria alternata的抑菌条件,并测定了其对百合叶斑病的田间防治效果。结果表明:TraT2A在200.00 mg/mL下对百合叶斑病菌A.alternata的抑菌作用较好,抑制率为72.12%;随着紫外光照时间的延长,TraT2A的抑菌活性逐渐降低,添加5.00 mg/mL的腐殖酸对TraT2A有较好的紫外保护作用,抑制率(69.01%)比不加紫外保护剂对照(29.37%)提高39.64%;添加0.17 mg/mL的辅助剂SP-4821A对TraT2A活性的提高效果最好,抑制率(95.13%)比TraT2A对照(69.16%)提高25.97%。按照优化后的配方:200 g TraT2A(质量浓度为200.00 mg/mL)+5 g腐殖酸(质量浓度为5.00 mg/mL)+0.17 g SP-4821A(质量浓度为0.17 mg/mL)+794.83 mL灭菌水,进行田间药效试验,结果发现其对百合叶斑病的防治效果为80.37%。该研究结果为进一步开发防治百合病害的TraT2A制剂提供了理论依据。In order to improve the inhibition activity and light stability of the protein extract TraT2A from the fermentation broth of Trichoderma atroviride T2 strain,the ultraviolet protective agents(ascorbic acid,humic acid)and the auxiliary agent(IE-08,SP-4821A and SP-4821B)were screened,the inhibition condition of TraT2A for Alternaria alternata were optimized through the colony growth rate test,and the field control efficacy on lily leaf spot disease was determined.The results showed that TraT2A had a good inhibitory effect on A.alternata at 200.00 mg/mL and the inhibitory rate was 72.12%.With the prolongation of the UV illumination time,the inhibition activity of TraT2A gradually decreases.The addition of 5.00 mg/mL humic acid had an excellent UV protection effect on TraT2A and its inhibition rate(69.01%)increased by 39.64%compared with that without a UV protection agent(29.37%).The addition of 0.17 mg/mL adjuvant of SP-4821A increased the inhibition activity of TraT2A most significantly and the inhibition rate(95.13%)increased by 25.97%compared with the TraT2A control(69.16%).According to the optimized formula,200 g TraT2A(the mass concentration was 200.00 mg/mL),5 g humic acid(the mass concentration was 5.00 mg/mL),0.17 g SP-4821A(the mass concentration was 0.17 mg/mL)and 794.83 mL sterilized water was mixed and the field control efficacy of the mixture was tested on lily leaf spot disease,the result showed that its field control efficacy was 80.37%.The research results provide a theoretical basis for the further development of TraT2A preparations for the control of lily diseases.

关 键 词：深绿木霉T2菌株 发酵液蛋白提取物 TraT2A 百合叶斑病菌 抑菌活性 田间防效 

分 类 号：S482.2[农业科学—农药学] TQ450.2[农业科学—植物保护]