多花水苋对苄嘧磺隆的抗性水平及靶标抗性机制  

Resistance level and target-site resistance mechanism to bensulfuron-methyl in Ammannia multiflora

作　　者：杨倩 卫甜 朱锦磊[1] 刘怀阿[1] 吕敏[1] YANG Qian;WEI Tian;ZHU Jinlei;LIU Huaia;LV Min(Institute of Agricultural Sciences for Lixiahe Region in Jiangsu,Yangzhou 225007,Jiangsu Province,China)

机构地区：[1]江苏里下河地区农业科学研究所,江苏扬州225007

出　　处：《农药学学报》2022年第4期798-804,共7页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金(31901904);江苏里下河地区农科所科研专项基金(SJ(21)301).

摘　　要：为明确水稻田杂草多花水苋Ammannia multiflora对乙酰乳酸合成酶(ALS)抑制剂类除草剂苄嘧磺隆的抗性水平和抗性分子机制,采用整株水平测定法,测定了采自江苏省扬州市田间的多花水苋疑似抗性种群(YZ-R)对苄嘧磺隆的抗性指数,并分析了YZ-R种群和相对敏感种群(YZ-S)多花水苋ALS酶对苄嘧磺隆的敏感性差异,同时比较了YZ-R和YZ-S种群ALS基因的核苷酸序列差异。结果表明:YZ-R种群多花水苋对苄嘧磺隆已表现出高水平抗性,其抗性指数(RI)为40.6;苄嘧磺隆对YZ-R种群ALS酶活性的抑制中浓度(I_(50))为0.087μmol/L,对YZ-S种群的I_(50)值为0.0028μmol/L,其抗性指数为31.1。通过PCR扩增获得了多花水苋ALS基因的部分序列,该序列包含了已报道的8个氨基酸突变位点。ALS基因序列比对分析发现,YZ-R种群多花水苋植株ALS基因第197位氨基酸由脯氨酸(CCT)突变为丝氨酸(TCT)。研究表明,ALS基因发生脯氨酸(Pro)-197-丝氨酸(Ser)的突变,导致多花水苋ALS酶对苄嘧磺隆的敏感性下降,是多花水苋YZ-R种群对苄嘧磺隆产生高水平抗性的主要原因。In order to investigate the resistance level and molecular mechanism of Ammannia multiflora Roxb.in paddy fields to acetolactate synthase(ALS)inhibiting herbicide bensulfuron-methyl,the resistance index(RI)of a putative resistant population YZ-R collected from Yangzhou city were performed by the whole-plant dose-response experiments.In vitro ALS enzyme sensitivity to bensulfuron-methyl and the nucleotide sequence of ALS gene from the susceptible population YZ-S and resistant population YZ-R were analyzed to characterize the target-site resistance mechanism.The whole-plant dose-response assays indicated that the YZ-R population had evolved a high level(RI=40.6)of resistance to bensulfuron-methyl.In addition,the I_(50) values of YZ-R and YZ-S were 0.087 and 0.0028μmol/L,respectively,and the RI was 31.1.The partial sequence of the target ALS gene was cloned by PCR,which contains eight reported amino acid mutation sites.Sequence alignment results showed the substitution of proline(Pro)197 by serine(Ser)in the YZ-R population.This study demonstrated that the reduced ALS enzyme sensitivity to bensulfuron-methyl resulted from the Pro-197-Ser substitution in the ALS gene,which was the main cause of the high-level resistance to bensulfuron-methyl in A.multiflora.

关 键 词：多花水苋 苄嘧磺隆 抗药性 靶标抗性 乙酰乳酸合成酶 基因突变 

分 类 号：S481.4[农业科学—农药学] TQ457[农业科学—植物保护]