基于HIV-1基因序列分析深圳口岸出入境人员与当地人群中CRF01_AE流行株的相关性  

The correlation on HIV-1 CRF01_AE strains in local population and entry-exit personnel at Shenzhen port based on HIV-1 gene sequences

作　　者：刘胜牙 陈震[3] 陈琳[4] 冯毅[5] 徐媛 赵锦[4] 李微 赵倩 陈琛 何翔[6] LIU Sheng-ya;CHEN Zhen;CHEN Lin;FENG Yi;XU Yuan;ZHAO Jin;LI Wei;ZHAO Qian;CHEN Chen;HE Xiang(Shenzhen International Travel Health Care Center,Shenzhen,Guangdong 518033,China;Shenzhen Academy of Inspection and Quarantine,Shenzhen,Guangdong 518010,China;Bao'an Center for Disease Control and prevention of Shenzhen,Shenzhen,Guangdong 518101,China;Shenzhen Center for Disease Control and prevention,Shenzhen,Guangdong 518073,China;National Center for AIDS/STD Control and Prevention,China CDC,Beijing 102211,China;Guangdong Provincial Institute of Public Health,Guangdong Provincial Center for Disease Control and prevention,Guangzhou,Guangdong 511430,China)

机构地区：[1]深圳国际旅行卫生保健中心(深圳海关口岸门诊部),广东深圳518033 [2]深圳市检验检疫科学研究院,广东深圳518010 [3]深圳市宝安区疾病预防控制中心,广东深圳518101 [4]深圳市疾病预防控制中心,广东深圳518073 [5]中国疾病预防控制中心性病艾滋病预防控制中心,北京102211 [6]广东省疾病预防控制中心广东省公共卫生研究院,广东广州511430

出　　处：《中国热带医学》2022年第3期195-200,共6页China Tropical Medicine

基　　金：国家自然科学基金项目(No.81102174);深圳市实施标准化战略资金资助项目(No.2015BZYZHY0102)。

摘　　要：目的 分析深圳口岸出入境人员与当地人群中流行的HIV-1 CRF01_AE毒株的基因相关性,为制定相应的防控政策提供指导依据。方法 以深圳口岸出入境人员与当地人群中HIV-1CRF01_AE毒株感染者为研究对象,获得两个人群中的HIV-1基因序列并判定基因型,分别应用传播网络和系统进化分析方法,研究两个人群中HIV-1CRF01_AE流行毒株的关联情况。结果 本研究获得2003—2014年途经深圳口岸的49例出入境人员HIV-1CRF01_AE感染者,以及111例2001—2014年深圳当地人员HIV-1 CRF01_AE感染者体内艾滋病病毒pol基因序列。深圳口岸出入境人员及当地人群中的HIV-1 CRF01_AE毒株传播网络模式图显示,6例中国大陆籍出境者感染的病毒与深圳当地流行毒株有较密切关系,2例香港籍、3例越南籍入境者体内毒株与当地流行毒株关系较密切。深圳口岸出入境人员及当地人群中的HIV-1 CRF01_AE近似最大似然法系统进化树显示,集中包含两个人群的HIV毒株基因序列所在的进化簇,并且可靠性检验系数在0.9以上的有五个。从系统进化树所处的位置,观察到1例马来西亚籍、1例韩国籍、3例香港籍的入境者以及4例中国大陆籍出境者体内毒株与深圳流行毒株相关。结论 深圳口岸出入境人员与当地人群中流行的HIV-1 CRF01_AE毒株之间可能存在关联,应持续加强对国境口岸出入境人员的HIV分子监测。Objective purpose of this study is to analyze its relatedness of circulating HIV-1 CRF01_AE strain in local population and Shenzhen port enter-exit personnel, so as to lay the foundation for the formulation of policies.Methods obtained from the entry-exit personnel and local people in Shenzhen, and the genotypes were determined. The transmission network and phylogenetic analysis were used to study the association of HIV-1 CRF01_AE in the two populations.ResultsIn this study, the pol gene sequences of 49 HIV-1 CRF01_AE infected entry-exit personnel at Shenzhen port from 2003 to 2014 and 111 HIV-1 CRF01_AE infected local population from 2001 to 2014 were obtained. The network pattern of HIV-1 CRF01_AE among entry-exit personnel at Shenzhen port and local population showed that the virus strains from six Chinese mainland exit personnel, two Chinese Hongkong immigrants and three Vietnamese immigrants had a close relationship with the local epidemic strains in Shenzhen. The phylogenetic tree of HIV-1 CRF01_AE among entry-exit personnel at Shenzhen port and local population showed that there were five evolutionary clusters containing the viral gene sequences from these two populations, and the reliability test coefficient of these clusters were more than 0.9. Based on the position of the phylogenetic tree, it was observed that a Malaysian immigrant, a Korean immigrant, three Chinese Hongkong immigrants, and four Chinese mainland exit personnel were associated with the local epidemic strains in Shenzhen.Conclusion association on circulating HIV-1 CRF01_AE strains the local population and the entry-exit personnel at Shenzhen port, and the HIV molecular surveillance on entry-exit personnel at border ports should be continuously strengthened.

关 键 词：HIV 基因 相关性 

分 类 号：R392.13[医药卫生—免疫学]