负载银和重组人碱性成纤维细胞生长因子的甲基丙烯酸酐化明胶水凝胶对兔深Ⅱ度烧伤创面的影响  

Effects of methacrylic anhydride gelatin hydrogel loaded with silver and recombinant human basic fibroblast growth factor on deep partial-thickness burn wounds in rabbits

作　　者：陈向军 吴兴 林欢欢 刘肇兴 刘沙 Chen Xiangjun;Wu Xing;Lin Huanhuan;Liu Zhaoxing;Liu Sha(Department of Burn Orthopaedics,the 969th Hospital of PLA Joint Logistic Support Force,Hohhot 010051,China)

机构地区：[1]解放军联勤保障部队第969医院烧伤整形科,呼和浩特010051

出　　处：《中华烧伤与创面修复杂志》2022年第7期640-649,共10页Chinese Journal of Burns And Wounds

基　　金：内蒙古自治区自然科学基金(2018MS08002)。

摘　　要：目的探讨负载银和重组人碱性成纤维细胞生长因子(rh-bFGF)的甲基丙烯酸酐化明胶(GelMA)水凝胶对兔深Ⅱ度烧伤创面的影响。方法采用实验研究方法。制备含不同浓度甲基丙烯酸酐(MA)的低浓度MA明胶(GelMA)材料、中浓度GelMA材料和高浓度GelMA材料,加入光引发剂后分别制得低浓度GelMA水凝胶、中浓度GelMA水凝胶和高浓度GelMA水凝胶。采用核磁共振波谱仪检测前述3种浓度GelMA材料的氢核磁共振谱并根据波谱图计算其取代度,采用场发射扫描电子显微镜(FESEM)检测前述3种浓度GelMA水凝胶的三维微观结构及孔径,样本数均为9。根据前述筛选出的MA浓度合成含10种浓度银的GelMA(含银GelMA)溶液,将每种浓度的含银GelMA溶液均分为3份,加入光引发剂后分别暴露于紫外光下持续20、25、35 s,制得相应的含银GelMA水凝胶。采用胶原酶降解法测定不同光交联时间含银GelMA水凝胶降解12、24、36、48 h的降解剩余率及彻底降解所需时长,样本数为5。测定前述筛选出光交联时间下含10种浓度银GelMA水凝胶对金黄色葡萄球菌的抑菌圈直径反映其抑菌能力,样本数均为5。以与含最低浓度银(即不含银)GelMA水凝胶抑菌圈直径相比有统计学意义的含银GelMA水凝胶为有抑菌活性。选取具有抑菌活性的且载药浓度最低的含银GelMA水凝胶,采用FESEM检测其三维微观结构及孔径,采用能谱仪检测其内部银元素的存在情况,样本数均为9。将冻干单纯GelMA水凝胶和冻干含银GelMA水凝胶分别浸没于磷酸盐缓冲液中24 h,通过称重法计算并比较2种水凝胶的溶胀率,样本数为5。根据预实验及前述实验结果,制备含银和rh-bFGF的GelMA水凝胶(简称复合水凝胶)。大体观察复合水凝胶的外观,并采用FESEM检测其三维微观结构与孔径。取30只4~6个月龄、雌雄各半日本大耳兔,在其背部制作深Ⅱ度烧伤创面。以兔头侧为基准,将脊柱左侧�Objective To investigate the effects of methacrylic anhydride gelatin(GelMA)hydrogel loaded with silver and recombinant human basic fibroblast growth factor(rh-bFGF)on deep partial-thickness burn wounds in rabbits.Methods The experimental research method was adopted.Low-concentration GelMA materials,medium-concentration GelMA materials and high-concentration GelMA materials containing different concentrations of methacrylic anhydride(MA)were prepared,after adding photoinitiator,low-concentration GelMA hydrogels,medium-concentration GelMA hydrogels,and high-concentration GelMA hydrogels were obtained,respectively.The nuclear magnetic resonance spectroscopy was performed to detect the hydrogen nuclear magnetic resonance spectra of the above-mentioned three concentrations of GelMA materials,and to calculate the degree of substitution according to the spectrum diagram.The three-dimensional microstructure and pore size of 3 types of above-mentioned GelMA hydrogels were detected by field emission scanning electron microscopy(FESEM),with 9 samples measured.According to the selected concentration of MA,ten kinds of solutions of GelMA with different concentration of silver(silver-containing GelMA)were synthesized,and the silver-containing GelMA solution of each concentration was divided into three parts,and then exposed to ultraviolet light lasting for 20,25,and 35 s,respectively.After adding photoinitiator,the corresponding silver-containing GelMA hydrogels were obtained.The residual degradation rate of silver-containing GelMA hydrogel with different photocrosslinking times was detected by collagenase degradation method at degradation of 12,24,36,and 48 h;and the time required for complete degradation was detected,and the sample number was 5.The inhibition zone diameter of GelMA hydrogel under above screened photocrosslinking times containing 10 concentrations of silver against Staphylococcus aureus was measured to reflect its antibacterial ability,and the sample numbers were all 5.The silver-containing GelMA hydrogel wi

关 键 词：波谱图 场发射扫描电子显微镜 水凝胶 核磁共振波谱仪 单因素方差分析 光交联 日本大耳兔 溶胀率 

分 类 号：R644[医药卫生—外科学]