针灸对湿热蕴肤型湿疹豚鼠皮肤屏障功能的影响  

Effect of acupuncture and moxibustion on skin barrier function in guinea pigs with eczema of skin damp-heat accumulation

作　　者：汪秀梅 武亦阁 贺乙 范丽娜 周光[1] WANG Xiu-mei;WU Yi-ge;HE Yi;FAN Li-na;ZHOU Guang(College of TCM,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学中医学院,乌鲁木齐830011

出　　处：《中国针灸》2022年第7期779-784,810,共7页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:81660763、81960825。

摘　　要：目的:观察针灸对湿热蕴肤型湿疹模型豚鼠皮肤角质层含水量、血清炎性因子及皮肤、肺、直肠组织水通道蛋白3(AQP_(3))表达的影响,探究针灸调控皮肤屏障功能的可能机制。方法:将24只雄性白化豚鼠随机分为空白组(6只)和造模组(18只),造模组采用2,4-二硝基氯苯(DNCB)诱导湿热蕴肤型湿疹模型,造模成功后的豚鼠随机分为模型组、西药组、针灸组,每组6只。西药组予氯雷他定片(0.8 mg/kg)灌胃,每日1次,连续7 d;针灸组针刺“肺俞”“脾俞”“曲池”“足三里”“血海”,留针15 min,同时于“肺俞”“足三里”施雀啄灸,每穴10 min,每日1次,连续7 d。分别于干预前后评定各组豚鼠湿疹面积及严重度指数(EASI)评分,应用皮肤测试仪检测皮肤角质层含水量(WCSC)及经皮肤水分流失量(TEWL);干预结束后,采用HE染色法观察各组豚鼠皮肤组织形态学变化,ELISA法测定各组豚鼠血清免疫球蛋白E(IgE)、白介素(IL)-4、IL-17含量,Western blot法测定各组豚鼠皮肤、肺、直肠组织AQP_(3)蛋白表达。结果:干预前,与空白组比较,其他各组豚鼠EASI评分、TEWL升高(P<0.01),WCSC降低(P<0.01);干预后,与模型组比较,西药组和针灸组豚鼠EASI评分、TEWL降低(P<0.05,P<0.01),WCSC升高(P<0.01)。空白组豚鼠表皮结构完整,真皮层纤维排列整齐;模型组豚鼠表皮角化过度,颗粒层、棘细胞层及基底层细胞增生,真皮层纤维排列紊乱,炎性细胞浸润;西药组、针灸组豚鼠形态学表现较模型组改善。与空白组比较,模型组豚鼠血清IgE、IL-17含量升高(P<0.01),血清IL-4含量及皮肤、肺、直肠组织AQP_(3)蛋白表达降低(P<0.01,P<0.05);与模型组比较,西药组、针灸组豚鼠血清IgE、IL-17含量降低(P<0.01),血清IL-4含量及针灸组豚鼠皮肤、肺、直肠组织AQP_(3)蛋白表达升高(P<0.01,P<0.05);与西药组比较,针灸组豚鼠血清IgE、IL-17含量升高(P<0.01),血清IL-4含量降低(P<0.01)Objective To observe the effect of acupuncture and moxibustion on the water content of stratum corneum(WCSC),expression of serum inflammatory factors and aquaporin 3(AQP_(3))in skin,lung and rectum in guinea pigs with eczema of skin damp-heat accumulation,and to explore the possible mechanism of acupuncture and moxibustion for regulating skin barrier function.Methods A total of 24 male albino guinea pigs were randomly divided into a blank group(n=6)and a modeling group(n=18).The guinea pigs in the modeling group were induced by 2,4-dinitrochlorobenzene(DNCB)to establish the eczema model of skin damp-heat accumulation.The guinea pigs with successful modeling were further randomly divided into a model group,a medication group and an acupuncture-moxibustion group,6 guinea pigs in each group.The guinea pigs in the medication group were treated with loratadine tablets(0.8 mg/kg)by gavage,once a day for 7 days;the guinea pigs in the acupuncture-moxibustion group were treated with acupuncture at"Feishu"(BL 13),"Pishu"(BL 20),"Quchi"(LI 11),"Zusanli"(ST 36)and"Xuehai"(SP 10);at the same time,moxibustion was applied at"Feishu"(BL 13)and"Zusanli"(ST 36),moxibustion intervention for 10 min and needle retaining for 15 min at each acupoint,once a day for 7 days.The eczema area and severity index(EASI)score was evaluated before and After intervention,and WCSC and trans-epidermal water loss(TEWL)were measured by skin tester.After intervention,The HE staining was used to observe the changes of skin histomorphology in each group;ELISA was used to measure the contents of serum immunoglobulin E(IgE),interleukin(IL)-4 and IL-17;Western blot was used to measure the protein expression of AQP_(3) in skin,lung and rectum.Results Before the intervention,compared with the blank group,the EASI scores and TEWL were increased in the remaining groups(P<0.01),and the WCSC was decreased(P<0.01).After the intervention,compared with the model group,the EASI scores and TEWL were decreased(P<0.05,P<0.01),and WCSC was increased(P<0.01)in the medicat

关 键 词：湿疹 湿热蕴肤型 针灸 表皮水分代谢 水通道蛋白3 

分 类 号：R246.7[医药卫生—针灸推拿学]