Schisandrin B Inhibits NLRP3 Inflammasome Pathway and Attenuates Early Brain Injury in Rats of Subarachnoid Hemorrhage  

作　　者：CHEN Song DING Yi-hang SHI Song-sheng TU Xian-kun 

机构地区：[1]Department of Neurosurgery,Fujian Medical University Union Hospital,Neurosurgery Research Institute of Fujian Province,Fuzhou 350001,China

出　　处：《Chinese Journal of Integrative Medicine》2022年第7期594-602,共9页中国结合医学杂志（英文版）

基　　金：Supported by the Natural Science Foundation of Fujian Province of China (No. 2020J011016);the Joint Funds for the Innovation of Science and Technology of Fujian Province (No. 2018Y9004);the Startup Fund for Scientific Research of Fujian Medical University (No. 2019QH1055)。

摘　　要：Objective: To determine whether Schisandrin B(Sch B) attenuates early brain injury(EBI) in rats with subarachnoid hemorrhage(SAH). Methods: Sprague-Dawley rats were divided into sham(sham operation), SAH, SAH+vehicle, and SAH+Sch B groups using a random number table. Rats underwent SAH by endovascular perforation and received Sch B(100 mg/kg) or normal saline after 2 and 12 h of SAH. SAH grading, neurological scores, brain water content, Evan’s blue extravasation, and terminal transferase-mediated dUTP nick end-labeling(TUNEL) staining were carried out 24 h after SAH. Immunofluorescent staining was performed to detect the expressions of ionized calcium binding adapter molecule 1(Iba-1) and myeloperoxidase(MPO) in the rat brain, while the expressions of B-cell lymphoma 2(Bcl-2), Bax, Caspase-3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3(NLRP3), apoptosis-associated specklike protein containing the caspase-1 activator domain(ASC), Caspase-1, interleukin(IL)-1β, and IL-18 in the rat brains were detected by Western blot. Results: Compared with the SAH group, Sch B significantly improved the neurological function, reduced brain water content, Evan’s blue content, and apoptotic cells number in the brain of rats(P<0.05 or P<0.01). Moreover, Sch B decreased SAH-induced expressions of Iba-1 and MPO(P<0.01). SAH caused the elevated expressions of Bax, Caspase-3, NLRP3, ASC, Caspase-1, IL-1β, and IL-18 in the rat brain(P<0.01), all of which were inhibited by Sch B(P<0.01). In addition, Sch B increased the Bcl-2 expression(P<0.01). Conclusion: Sch B attenuated SAH-induced EBI, which might be associated with the inhibition of neuroinflammation, neuronal apoptosis, and the NLRP3 inflammatory signaling pathway.

关 键 词：schisandrin B subarachnoid hemorrhage early brain injury inflammation neuronal apoptosis nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]