褐家鼠背毛和腹毛红外光谱分析  

Infrared spectrum analysis of the back and abdominal hairs of Rattus norvegicus

作　　者：杨文建 周思宇 苏航 万祥旭 金志民[1] YANG Wen-jian;ZHOU Si-yu;SU Hang;WAN Xiang-xu;JIN Zhi-min(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《生物技术》2022年第3期321-324,共4页Biotechnology

基　　金：黑龙江省自然科学基金项目(“气候变化下张广才岭森林啮齿动物群落格局动态及对林业影响研究”,LH2020C071);(“张广才岭生态区啮齿动物食物资源利用模式对农林植物的影响”,SS2021C006)。

摘　　要：[目的]了解褐家鼠(Rattus norvegicus)背毛和腹毛的红外光谱特征,分析其异同点。[方法]使用傅里叶变换红外光谱(FT-IR)仪对背毛和腹毛的粉末进行检测,根据得到的红外光谱和二阶导数谱分析不同部位体毛的特征。[结果]结果表明,褐家鼠背毛和腹毛均能反映出该物种的特征性成分,背毛和腹毛的红外图谱基本一致,如背毛和腹毛均在1561、1637、622 cm^(-1)位置形成了明显的吸收峰,在3397~3494 cm^(-1)范围内表现了明显的N-H基团的伸缩振动峰。但二阶导数谱能明显地反映出背毛和腹毛之间的差异,如腹毛在1626 cm^(-1)有一个峰强较小的峰,背毛表现不明显;腹毛在1773 cm^(-1)处有一个明显的单峰,而背毛在1768~1775 cm^(-1)范围内由两个峰强和峰形相似的单峰形成一个封底较宽的较小的W形图谱。[结论]红外光谱可以反映褐家鼠体毛的特征性成分,其不同部位的体毛特征基本一致,但依据红外光谱1234~1258 cm^(-1)范围内和二阶导数谱1626~1775 cm^(-1)范围内的图谱可以区分体毛处于的不同部位。利用该特征可以对褐家鼠进行鉴定并对鸮形目(Strigiformes)鸟类的食性进行分析。[Objective]To understand the infrared spectrum characteristics of back and abdominal hair of Rattus norvegicus and analyze the similarities and differences between them.[Method]Fourier Transform infrared spectroscopy(FT-IR)was used to detect the powder of back hair and abdominal hair,and the characteristics of body hair in different parts were analyzed according to the infrared spectrum and second derivative spectrum obtained.[Result]The results showed that the dorsal hair and abdominal hair of Rattus norvegicus could reflect the characteristic components of this species.The infrared patterns of dorsal hair and abdominal hair were basically the same.For example,the dorsal hair and abdominal hair both formed obvious absorption peaks in 1561,1637,622 cm^(-1),and showed obvious stretching vibration peaks of N-H groups in 3397-3494 cm^(-1).But the second derivative spectrum can obviously reflect the difference between the back hair and abdominal hair,such as abdominal hair in 1626 cm^(-1)has a small peak strength,the back hair is not obvious;The abdominal hair has a distinct single peak in 1773 cm^(-1),while the back hair has two similar single peaks in 1768-1775 cm^(-1),forming a smaller W-shaped pattern with a wider back cover.[Conclusion]The infrared spectrum can reflect the characteristic components of the hair of Rattus norvegicus,and the hair characteristics of different parts are basically the same,but the different parts of the hair can be distinguished according to the infrared spectrum in the range of 1234-1258 cm^(-1)and the second derivative spectrum in the range of 1626-1775 cm^(-1).The characteristics can be used to identify Rattus norvegicus and analyze the feeding habits of Strigiformes birds.

关 键 词：褐家鼠 鸮形目 背毛 腹毛 傅里叶红外光谱 二阶导数谱 

分 类 号：Q95-336[生物学—动物学]