加味香连丸HPLC指纹图谱和11个成分定量分析  

Study on HPLC Fingerprint and Quantification of 11 Components in Jiawei Xianglian Pills

作　　者：钟国男 张余芳[1] 林冠 钟小丽 ZHONG Guo-nan;ZHANG Yu-fang;LIN Guan;ZHONG Xiao-li(Pharmacy Department of Haikou Maternal and Child Health Care Hospital,Haikou 570203,China)

机构地区：[1]海口市妇幼保健院药剂科,海南海口570203

出　　处：《食品与药品》2022年第4期344-351,共8页Food and Drug

摘　　要：目的建立加味香连丸高效液相色谱(HPLC)指纹图谱,测定11个有效成分含量。方法采用HPLC法,使用Agilent SB-C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.05%磷酸水溶液为流动相,梯度洗脱,流速为1.0 ml/min,检测波长225,230,280 nm,柱温30℃,进样量10μl。选取黄芩苷为参照峰,并使用中药色谱指纹图谱相似度评价系统对指纹图谱进行相似性评价。结果建立加味香连丸指纹图谱,15批加味香连丸指纹图谱与对照指纹图谱的相似度均大于0.99;共标定29个共有峰,对其中11个色谱峰进行了定性定量研究,各项方法学考察结果均符合测试要求。结论所建立的HPLC指纹图谱和多成分含量分析方法可用于加味香连丸的质量控制。Objective To establish the HPLC fingerprint and determine the contents of 11 compounds in Jiawei Xianglian Pills.Methods HPLC analysis was performed on an Agilent SB-C_(18) column(250 mm×4.6 mm,5μm),using acetonitrile-0.05%phosphoric acid at a flow rate of 1.0 ml/min as the mobile phase of gradient elution.The detection wavelength was set at 225,230 and 280 nm,the column temperature was set at 30℃,and the sample size was 10μl.By selection of baicalin as reference peak,the HPLC fingerprint was analyzed and the similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine.Results HPLC fingerprint of Jiawei Xianglian Pills was established,and the similarities between the fingerprints of 15 batches of Jiawei Xianglian Pills and the control fingerprint were all greater than 0.99.29 common peaks were identified,among which,11 peaks were qualitatively and quantitatively studied.The methodological investigation results were all in accordance with the testing requirements.Conclusion HPLC fingerprint and multi-component analysis method established can be used for quality control of Jiawei Xianglian Pills.

关 键 词：加味香连丸 指纹图谱 相似度分析 含量测定 

分 类 号：R917[医药卫生—药物分析学]