P物质/NK1受体对实验性胃溃疡大鼠胃肠动力的调节  

The Regulation of SP/NK1 Receptor on Gastrointestinal Motility in Experimental Gastric Ulcer Rats

作　　者：王舒怡 徐进 葛婉悦 王佑华 张玉平[1] 吴靖芳[1] WANG Shu-yi;XU Jin;GE Wan-yue;WANG You-hua;ZAHNG Yu-ping;WU Jing-fang(Morphology Laboratory,School of Basic Medicine,Hebei North University,Zhangjiakou,075000,China;School of Basic Medicine,Hebei North University,Zhangjiakou,075000,China)

机构地区：[1]河北北方学院,基础医学院形态学实验室,张家口075000 [2]河北北方学院,基础医学院,张家口075000

出　　处：《神经药理学报》2021年第6期10-16,共7页Acta Neuropharmacologica

基　　金：国家大学生创新基金项目(No.201910092002)。

摘　　要：目的:通过检测大鼠实验性胃溃疡愈合过程中胃肠功能及P物质(substance P,SP)表达改变,探讨SP/NK1受体(neurokinin1,NK1)通路对胃溃疡离体幽门平滑肌条收缩功能的影响及机制。方法:62只SD大鼠分为正常组(6只)、盐水对照组(24只)和溃疡组(32只)。采用常规冰乙酸腐蚀法制备大鼠乙酸性胃溃疡模型并测量溃疡面积;免疫组化染色观察SP的表达。甲基纤维素酚红溶液灌胃观察胃排空率和肠推进功能;张力换能器及多通道生理信号采集处理系统观察SP及NK1受体拮抗剂对离体胃幽门环形平滑肌条收缩反应性的影响。结果:溃疡组术后4 d形成呈典型的“环堤征”,10~14 d溃疡逐渐愈合,28 d溃疡处形成瘢痕组织。免疫组化结果显示:SP阳性细胞主要分布于黏膜胃底腺细胞、黏膜下层和肌层,溃疡4、10和14 d SP积分光密度值明显低于正常组和对照组(P<0.05,P<0.01)。溃疡组的胃排空率明显低于正常组和盐水组(P<0.05),盐水组胃排空率与正常组相比无统计学意义。幽门环形肌条收缩功能显示:溃疡组不同时相的肌肉收缩幅度与正常组相比均有不同程度降低(P<0.01);加入SP后,胃幽门环形肌收缩幅度增加,在加入NK1受体拮抗剂后,收缩幅度、收缩频率大幅度下降(P<0.05,P<0.01)。结论:胃溃疡时胃肠运动功能显著降低,其机制与SP/NK1受体信号通路受损有关。Objective:To investigate the effect of substance P(SP)/NK1 receptor pathway on contractile function of isolated pyloric smooth muscle strips by detection gastrointestinal function and SP expression during experimental gastric ulcer.Methods:62 SD rats were divided into normal group(n=6),saline control group(n=24)and ulcer group(n=32).Gastric ulcer model were used acetic acid-induced in rats,and the ulcer area was measured.Gastric emptying rate and intestinal propulsive function were observed by intragastric administration of methyl cellulose.The effects of SP and NK1 recepter antagonist on the contractile reactivity of isolated pyloric annular smooth muscle strips with a tension transducer and a multi-channel physiological signal acquisition and processing system.Results:The typical“Ring dike sign”was formed on the 4^(th) day,the ulcer gradually healed from the 10^(th) to 14^(th) day,and the scar tissue was formed on the 28^(th) day.Immunohistochemical results showed that SP positive cells were mainly distributed in fundic gland cells,submucosa and myometrium of gastric mucosa.The integral optical density of SP in ulcer at the 4^(th),10^(th) and 14^(th) days was significantly lower than that in normal and control groups(P<0.05,P<0.01).Gastric emptying rate in ulcer group was significantly lower than that in normal group and saline group(P<0.05).The contractile amplitude of pyloric annular muscle strips in ulcer group was decreased in different degrees compared with that in normal group(P<0.01),when NK1 receptor antagonist was added,the contraction amplitude and frequency decreased significantly(P<0.05,P<0.01).Conclusion:The mechanism of decreased gastrointestinal motility in gastric ulcer is related to SP/NK1 receptor signal pathway.

关 键 词：胃溃疡 NK1受体 P物质 动力 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学]