平地木化学成分及含量分析  

Analysis of Chemical Composition and Content of Ardisia japonica(Thunb) Blume

作　　者：张冠腾 庞策 左亚奇 甄攀[1] ZHANG Guan-teng;PANG Ce;ZUO Ya-qi;ZHEN Pan(Hebei North University,Zhangjiakou,075000,China)

机构地区：[1]河北北方学院,张家口075000

出　　处：《神经药理学报》2021年第6期35-39,共5页Acta Neuropharmacologica

基　　金：河北省高等学校科学技术研究项目(No.ZD2016016),2018年度河北北方学院一般项目(No.YB2018030)。

摘　　要：目的:研究平地木的化学成分,测定平地木中化学成分的含量。方法:利用硅胶、D101大孔吸附树脂、C18等色谱方法分离,制备色谱仪纯制,用质谱(mass spectrometry,MS)、紫外光谱(ultraviolet and visible spectrum,UV)、红外图谱(infrared spectroscopy,IR)、核磁共振谱(nuclear magnetic resonance spectroscopy,NMR)等波谱方法鉴定其分子结构。以自制的化合物2单体为对照品,用高效液相色谱法(high performance liquid chromatography,HPLC)测定平地中化合物2的含量。结果:从平地木中发现四种化合物,分别为2-甲基-5-[10′(顺)-十五烯基]-间苯二酚(1),2-甲基-5-[8′(顺)-十三烯基]-间苯二酚(2),2-甲基-5-十三烷基间苯二酚(3),2-甲基-5-十五烷基间苯二酚(4)。平地木根、茎、叶中化合物2的含量分别为16.79 mg·g、19.39 mg·g和24.59 mg·g。结论:化合物1为新化合物,化合物3和化合物4为首次从平地木中发现。Objective:To study the chemical constituents and determine the content of the chemical constituents in Ardisia japonica(Thunb) Blume. Methods:Separation by silica gel,D101 macroporous adsorption resin,C18 and other chromatographic methods. Purification of preparative chromatograph. Identification of molecular structure by MS,UV,IR,NMR and other spectroscopic methods. The content of compound 2 in Ardisia japonica(Thunb) Blume was determined by HPLC with the homemade compound 2 as the control substance. Results: Four compounds were found from Ardisia japonica(Thunb) Blume,2-methyl-5-[10’(Z)-pentadecenyl]-resorcinol(1),2-methyl-5-[8’(Z)-tridecylene]-resorcinol(2),2-methyl-5-tridecyl-resorcinol(3),2-methyl-5-pentadecyl-resorcinol(4) respectively. The content of compound 2 in roots,stems and leaves of Ardisia japonica(Thunb) Blume was 16.79 mg·g、19.39mg·gand 24.59 mg·grespectively. Conclusion: Compound 1 was a new compound.Compounds 3 and 4 were first discovered from Ardisia japonica(Thunb) Blume.

关 键 词：紫金牛 平地木 化学成分 HPLC 

分 类 号：R284[医药卫生—中药学]