基于RAPD技术分析盐胁迫对大豆幼苗根系DNA的损伤  

DNA damage of roots in soybean seedlings under salt stress detected by random amplified polymorphic (RAPD) analysis

作　　者：陈素玉 赵强 于高波[1] 任春元[1] 张玉先[1] CHEN Su-yu;ZHAO Qiang;YU Gao-bo;REN Chun-yuan;ZHANG Yu-xian(College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163316,Heilongjiang,China;National Engineering Technology Research Center for Coarse Cereals,Daqing 163316,Heilongjiang,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163316 [2]国家杂粮工程技术研究中心,黑龙江大庆163316

出　　处：《生态学杂志》2022年第7期1441-1447,共7页Chinese Journal of Ecology

基　　金：黑龙江省农垦总局重点科研计划项目(HKKY190206-01);财政部和农业农村部国家现代农业产业技术体系(CARS-04-01A)资助。

摘　　要：盐胁迫是自然界中影响作物生长和产量形成的非生物胁迫因素之一。盐胁迫能够诱导作物产生DNA损伤,从而对作物产生细胞毒性和遗传毒害。本研究选取12对随机引物,使用随机扩增多态基因(RAPD)技术,分析0~150 mmol·L^(-1) NaCl处理5天对大豆幼苗根系造成的DNA损伤情况。结果表明:与对照组相比,NaCl处理能够显著减少大豆幼苗的根长、根鲜重、根干重、幼苗鲜重和幼苗干重。同时,对大豆幼苗根系造成显著的DNA损伤,并且损伤的程度随NaCl浓度的升高而加重。不同浓度NaCl胁迫下,12个随机引物共扩增出RAPD多态性片段数量为72~89个。其中,使用随机引物2和9扩增出的RAPD多态性片段条带清晰,且数量大于10个,可作为利用RAPD方法分析盐(NaCl)胁迫造成大豆DNA损伤的合适引物。Salt stress is one of the abiotic stresses in nature affecting crop growth and yield.Salt stress can cause DNA damage,resulting in cytotoxicity and genetic toxicity in crops.In this study,12 pairs of random primers were selected for random amplification of polymorphic DNA(RAPD)analysis to evaluate DNA damage in roots of soybean seedlings treated with 0-150 mmol·L^(-1) NaCl for five days.Our results showed that NaCl treatment significantly decreased root length,fresh and dry root weight,and fresh and dry weight of seedlings.Under salt stress,severe DNA damage was detected in roots of soybean seedlings,with damage degree being positively correlated with NaCl concentration.Under salt stress with different NaCl concentrations,the numbers of RAPD fragments amplified by 12 random primers ranged from 72 to 89.Among them,amplification using random primers 2 and 9 generated more than 10 clear bands of RAPD polymorphic fragments.Therefore,our results showed that random primers 2 and 9 could be used for RAPD assays to analyze DNA damage in soybean seedlings under salt stress.

关 键 词：RAPD 随机引物 遗传毒害 

分 类 号：S565.1[农业科学—作物学]