基于熵权法多指标优化栀子中环烯醚萜类成分的提取工艺  

Optimization of extraction process of iridoids from Gardenia based on entropy weight method and multiple indexes

作　　者：付庆 肖学凤[1] 周钰通 杨冰 岳钰茗 陈建宇[5] 单淇[2,3,4] 周福军 FU Qing;XIAO Xue-feng;ZHOU Yu-tong;YANG Bing;YUE Yu-ming;CHEN Jian-yu;SHAN Qi;ZHOU Fu-jun(Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Tianjin Institute of Pharmaceutical Research,Tianjin 300071,China;Tianjin Key Laboratory of Quality Marker of Traditional Medicine,Tianjin 300071,China;State Key Laboratory of Drug Delivery and Pharmacokinetics,Tianjin Institute of Pharmaceutical Research,Tianjin 300071,China;Pesticide National Engineering Research Center,Nankai University,Tianjin 300071,China)

机构地区：[1]天津中医药大学,天津300193 [2]天津药物研究院,天津300301 [3]天津市中药质量标志物重点实验室,天津300301 [4]天津药物研究院释药技术与药代动力学国家重点实验室,天津300301 [5]南开大学农药国家工程研究中心,天津300071

出　　处：《中国新药杂志》2022年第13期1265-1272,共8页Chinese Journal of New Drugs

基　　金：国家“重大新药创制”科技重大专项资助项目(2017ZX09301062)。

摘　　要：目的:联用熵权法(entropy weight method)与正交实验设计(orthogonal experimental design)法,多指标筛选栀子中环烯醚萜类成分的最佳提取工艺。方法:基于栀子HPLC指纹图谱构建的基础上,采用HPLC法测定栀子中去乙酰车叶草苷酸甲酯、京尼平龙胆双糖苷、栀子苷、西红花苷Ⅰ、西红花苷Ⅱ的含量,并以此5种指标成分的质量分数和浸膏率为评价指标,联用熵权法和正交实验设计法对栀子中环烯醚萜类成分的提取工艺参数进行优化。结果:根据综合评价评分(M)结果,确定了栀子中环烯醚萜类成分的最佳提取工艺参数为10∶8∶8倍量的水,回流提取3次,每次2 h。3批平行验证M评分分别为98.07,98.56,98.77,RSD为0.36%。结论:联用熵权法和正交实验设计法对栀子中环烯醚萜类成分的提取工艺参数进行优化,科学可行,简单易于操作,节约成本,重复性和稳定性好。Objective:The parameters of the extraction process of iridoids from Gardenia were multi-objectively optimized using combining the entropy weight method with orthogonal experimental design.Methods:Based on the construction of the HPLC fingerprint of Gardenia,HPLC was employed to determine the content of deacetylasperulosidic acid methyl ester,genipin gentioside,geniposide,crocinⅠ,and crocinⅡin Gardenia,the mass fraction and extract rate of which were chosen as comprehensive evaluation indexes.The extraction process parameters of iridoids from Gardenia were optimized by combining the entropy weight method with the orthogonal experiment design.Results:According to the result of comprehensive score,the optimal extraction process was refluxing for 3 times,2 hours each time,water volume 10:8:8 to gardenia weight,respectively.The comprehensive scores of the three batches of parallel verification were 98.07,98.56,98.77,respectively,and the RSD was 0.36%.Conclusion:The entropy weight method and orthogonal experiment design method were combined to optimize the extraction process parameters,which are scientific,feasible,repeatable,cost-saving and easy to operate.

关 键 词：熵权法 正交实验设计 提取工艺 栀子 环烯醚萜 

分 类 号：R943[医药卫生—药剂学]