炎宁糖浆与5种临床常用药物对急性肺损伤大鼠的改善作用  

Effects of Yanning Syrup and five common clinically used drugs on acute lung injury improvements in rats

作　　者：魏丹妮 彭颖[1] 马萍 李晓波[1] WEI Dan-ni;PENG Ying;MA Ping;LI Xiao-bo(College of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《中成药》2022年第7期2138-2145,共8页Chinese Traditional Patent Medicine

摘　　要：目的 比较炎宁糖浆与5种临床常用药物对急性肺损伤大鼠的改善作用。方法 采用腹腔注射脂多糖(8 mg/kg)诱导急性肺损伤模型,观察肺组织病理学,检测大鼠体质量和死亡率、肺湿干重比、血常规、血清及肺组织7种炎症因子(IL-2、IL-4、IL-6、IL-10、IL-17A、IFN-γ、TNF-α)水平,考察炎宁糖浆与抗生素(阿莫西林、阿奇霉素、左氧氟沙星)、中成药(蒲地蓝消炎口服液、连花清瘟胶囊)对大鼠的改善作用。结果 炎宁糖浆可升高大鼠体质量(P<0.05,P<0.01),减轻肺组织病理损伤、肺水肿(P<0.05,P<0.01),提高血小板密度(P<0.05),调节免疫抑制状态下大鼠血清促炎因子(IFN-γ、IL-2、IL-6)和抗炎因子(IL-4、IL-17A)水平(P<0.05);阿奇霉素能升高大鼠体质量(P<0.01),减轻肺组织病理损伤,降低大鼠血液中间细胞和粒细胞密度(P<0.05),上调大鼠血清IL-4、IL-10水平(P<0.05);左氧氟沙星、蒲地蓝消炎口服液和连花清瘟胶囊对大鼠部分指标发挥调控作用,而阿莫西林对大鼠各指标均无明显作用。结论 阿奇霉素较阿莫西林、左氧氟沙星更有效地减轻急性肺损伤大鼠症状。中成药(炎宁糖浆、蒲地兰消炎口服液和连花清瘟胶囊)均具有一定改善作用但特点不同,炎宁糖浆在改善大鼠肺水肿、血小板减少、炎症因子分泌失调等方面发挥较好的作用。AIM To compare the effects of Yanning Syrup and five common clinically used drugs on acute lung injury improvements in rats.METHODS Upon the rat models of acute lung injury established by intraperitoneal injection of lipopolysaccharide(8 mg/kg),the effects of Yanning Syrup,common antibiotics(amoxicillin,azithromycin and levofloxacin),Chinese patent medicines(Pudilan Anti-inflammatory Oral Liquid and Lianhua Qingwen Capsules)were investigated and compared.The rats had their body weights and mortality,lung pathology,lung wet/dry ratio and blood routine,as well the levels of seven inflammatory factors(IL-2,IL-4,IL-6,IL-10,IL-17 A,IFN-γ,TNF-α)in serum and lung tissue detected.RESULTS Yanning Syrup increased the rats’body weights(P<0.05,P<0.01),reduced their pulmonary pathological damage and edema(P<0.05,P<0.01),increased the platelet density(P<0.05),and regulated the levels of pro-inflammatory factors(IFN-γ,IL-2 and IL-6)and serum anti-inflammatory factors(IL-4 and IL-17 A)(P<0.05).Azithromycin increased the rats’body weights(P<0.01),reduced their pulmonary pathological damage,the density of blood intermediate cells and granulocytes(P<0.05),and up-regulated their serum levels of IL-4 and IL-10(P<0.05).Levofloxacin,Pudilan Anti-inflammatory Oral Liquid and Lianhua Qingwen Capsules involved in the regulatory process as revealed by the changes in some indices levels,in contrast to amoxicillin’s non-effects.CONCLUSION Compared with amoxicillin and levofloxacin,azithromycin holds its priority in relieving rats’symptoms of acute lung injury more effectively.All of the Chinese patent medicines(Yanning syrup,Pudilan Anti-inflammatory Oral Liquid and Lianhua Qingwen Capsules)present certain improvement efficacy though with different properties.Yanning syrup demonstrates better improvement efficacy in terms of pulmonary edema,thrombocytopenia,and secretion disorders of inflammatory factors in rats.

关 键 词：炎宁糖浆 急性肺损伤 抗生素 中成药 脂多糖 炎症因子 免疫抑制 

分 类 号：R285.5[医药卫生—中药学]