蒙药蓝刺头对去卵巢骨质疏松大鼠TGF-β1、UI、BMD的作用  

Influence of Mongolian Medicine Lancitou on TGF-β1,UI and BMD of Ovariectomized Rats with Osteoporosis

作　　者：赵军 师建平 ZHAO Jun;SHI Jianping(Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine,Hohehot 010020,China;College of TCM,Inner Mongolia Medical University)

机构地区：[1]内蒙古自治区中医医院,内蒙古呼和浩特010020 [2]内蒙古医科大学中医学院

出　　处：《西部中医药》2022年第7期16-19,共4页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30560165);国家中医药管理局中医药传承与创新“百千万人才工程”项目(J20184832009);第四批全国中医(临床、基础)优秀人才研修项目(J20184832009);内蒙古医科大学“科技百万工程”联合项目[YKD2017KJBW(LH)064];内蒙古自治区第三批老蒙医药中医药专家学术经验继承项目(内卫计蒙中字[2019]126);内蒙古自治区草原英才创新团队“中医治未病”项目(CYYCTD2018);内蒙古自治区自然科学基金(2009MS1134)。

摘　　要：目的:观察蒙药蓝刺头对去卵巢骨质疏松(osteoporosis,OP)模型大鼠血清白细胞介素1(interleukin-1,IL-1)、股骨头转化生长因子β_(1)(transforming growth factor-β_(1),TGF-β_(1))、骨密度(bone mineral density,BMD)、子宫指数(uterus index,UI)的调节作用。方法:制备去卵巢OP大鼠模型,90天后应用蒙药蓝刺头高、中、低浓度及强骨胶囊、己烯雌酚分别灌胃,采血检测血清IL-1,免疫组化染色检测大鼠左侧股骨头TGF-β_(1);显微CT分析大鼠右侧股骨BMD;计算子宫指数。结果:大鼠去势后子宫缩小,内膜层变薄,腺体上皮、内膜上皮变薄,腺腔直径缩小。1)与假手术组比较,模型组IL-1表达增高,左股骨头TGF-β_(1)、右股骨近端干骺端BMD降低(P<0.01);与模型组比较,蒙药蓝刺头中剂量组、己烯雌酚组、强骨胶囊组血清IL-1表达降低,左股骨头TGF-β_(1)、右股骨近端干骺端BMD增高(P<0.05)。2)UI模型组与假手术组比较差异显著(P<0.01),模型组与蒙药蓝刺头低剂量组比较差异无统计学意义(P>0.05);假手术组与蒙药蓝刺头高、中剂量组及强骨胶囊组、己烯雌酚组比较差异无统计学意义(P>0.05);蒙药蓝刺头与强骨胶囊及己烯雌酚各指标比较差异无统计学意义(P>0.05)。结论:一定剂量蒙药蓝刺头能有效防治PMOP,缓解去势所致副作用,可为运用蒙医“补暖补精-清镇赫依-固骨质壮骨”理论防治PMOP提供实验依据。Objective:To observe the regulatory effects of Mongolian medicine Lancitou on IL-1,TGF-β_(1),BMD and UI in ovariectomized rat models with OP.Methods:After establishing ovariectomized models with postmenopausal osteoporosis(PMOP),they were drenched with high,moderate and low doses of Mongolian medicine Lancitou,Qianggu capsules and diethylstilbestrol(DES)for 90 days respectively,to detect the level of IL-1 after drawing blood,to measure the expressions of TGF-β_(1) in left femoral head by using immumohistochemical staining;to analyze BMD in right femoral head by using micro-CT;to calculate UI.Results:After castration,the uterus shrank,endometrium,glandular epithelium and intimal epithelium become thinner,lumens diameter reduced.1)Compared with sham operation group,the expressions of IL-1 in the model group increased,the expressions of TGF-β_(1) in left femoral head and BMD in proximal epiphysis of the right femur reduced(P<0.01);compared with the model group,the expressions of IL-1 lowered in moderate dose groups of Mongolian medicine Lancitou,DES group and Qianggu capsules group,TGF-β_(1) in left femoral head and BMD in proximal epiphysis of the right femur increased(P<0.05).2)The difference was statistically significant when UI model group was compared with sham operation group(P<0.01),the difference had no statistical meaning when the model group was compared with low dose of Mongolian medicine Lancitou(P>0.05);the difference had no statistical meaning when sham operation group was compared with high,moderate dose groups of Mongolian medicine Lancitou,Qianggu capsules group and DES group(P>0.05);the difference had no statistical meaning in the indexes of Mongolian medicine Lancitou,Qianggu capsules and DES(P>0.05).Conclusion:Some dose of Mongolian medicine Lancitou could effectively prevent and treat PMOP,alleviate the adverse reaction induced by castration,it could provide experimental reference for the prevention and treatment of PMOP by using the theory“warming and supplementing essence-clearing and ca

关 键 词：骨质疏松症 绝经后 白细胞介素1 转化生长因子β1 子宫指数 蒙药蓝刺头 

分 类 号：R592[医药卫生—老年医学]