抗纤软肝颗粒对肝纤维化小鼠肠道黏膜通透性的影响  

Influence of Anti-fibrosis Liver-softening Granules on Intestinal Mucosal Permeability in Hepatic Fibrosis Mice

作　　者：徐俊 戚璐[1] 许杰[1] 李硕 程良斌[1] XU Jun;QI Lu;XU Jie;LI Shuo;CHENG Liangbin(Hubei University of Chinese Medicine,Wuhan 430061,China)

机构地区：[1]湖北中医药大学,湖北武汉430061

出　　处：《西部中医药》2022年第7期27-30,共4页Western Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局国家中医临床研究基地业务建设科研专项(JDZX2015179)。

摘　　要：目的:观察抗纤软肝颗粒对CCl_(4)诱导肝纤维化小鼠肠道黏膜通透性的影响。方法:将35只C57BL/6雄性小鼠随机分为正常组10只,造模组25只,造模组采用CCl_(4)皮下注射诱导肝纤维化小鼠模型,经病理学验证筛选出成功肝纤维化模型小鼠20只,将20只造模成功小鼠随机分为模型组和抗纤软肝颗粒治疗组(简称治疗组)各10只。治疗组灌胃抗纤软肝颗粒[3.9 g/(kg·d)],模型组灌胃等体积生理盐水,干预8周后对小鼠肝组织进行HE和Masson染色,免疫组化法检测结肠组织Occludin、Claudin-1、ZO-1蛋白表达,实时荧光定量PCR(RT-qPCR)法检测结肠Occludin、Claudin-1、ZO-1 mRNA表达。结果:HE染色和Masson染色显示模型组小鼠肝组织中胶原纤维广泛增生,纤维间隔相互连接,假小叶形成,治疗组小鼠肝脏组织纤维化程度较模型组减轻;小鼠结肠Occludin、Claudin-1、ZO-1及其mRNA表达模型组均低于正常组(P<0.05),治疗组较模型组上调(P<0.05)。结论:抗纤软肝颗粒抗肝纤维化作用可能与其上调结肠组织中Occludin、Claudin-1、ZO-1蛋白及其基因表达水平相关。Objective:To observe the effects of anti-fibrosis liver-softening granules on intestinal mucosal permeability in mice suffering CCl_(4)-induced hepatic fibrosis.Methods:A total of 35 C57BL/6 mice were randomized into ten mice in the normal group and 25 ones in the modeling group,the modeling group were prepared into hepatic fibrosis mice models by hypodermic injection of CCl_(4),20 successful mice models with hepatic fibrosis were screened out via pathological verification,randomized into the model group and the treatment group of anti-fibrosis liver-softening granules(abbreviated as the treatment group),with ten mice in each group.The treatment group was drenched with anti-fibrosis liver-softening granules[3.9 g/(kg·d)],and the model group with the same volume of normal saline,after eight weeks of intervention,liver tissues were stained by HE and Masson,immunohistochemical method was applied to detect the expressions of Occludin,Claudin-1 and ZO-1 protein in colon tissue,RT-qPCR method was used to measure the expressions of Occludin,Claudin-1 and ZO-1 mRNA.Results:HE and Masson staining displayed that collagen fibers were extensively proliferated in liver tissue of the mice in the model group,fibrous septa are interconnected,and pseudolobule were formed.The degrees of fibrosis in liver tissue of the mice in the treatment group were relieved compared with these of the model group;the expressions of Occludin,Claudin-1 and ZO-1 and their mRNA in colon of the mice in the model group were lower than these of the normal group(P<0.05),the treatment group was up-regulated compared with the model group(P<0.05).Conclusion:Anti liver fibrosis effects of anti-fibrosis liver-softening granules might be related to its up-regulation of the expressions of Occludin,Claudin-1 and ZO-1 protein and mRNA in colon tissue.

关 键 词：肝纤维化 肠道 紧密连接蛋白 抗纤软肝颗粒 小鼠 

分 类 号：R285.5[医药卫生—中药学]