模拟水分胁迫对多年生黑麦草种子萌发和生理调控的影响  

Simulated water stress on seed germination and physiological regulation of perennial ryegrass

作　　者：刘亚西 周杨 李杨 刘金平[1] 王丹 LIU Yaxi;ZHOU Yang;LI Yang;LIU Jinping;WANG Dan(Key Laboratory of Southwest China Wildlife Resources Conservation(Ministry of Education),College of Life Science/China West Normal University,Nanchong 637009,Sichuan,China)

机构地区：[1]西华师范大学生命科学学院/西南野生动植物资源保护省部共建教育部重点实验室,四川南充637009

出　　处：《草业科学》2022年第7期1383-1390,共8页Pratacultural Science

基　　金：西华师范大学博士科研基金(412837);四川省一流本科专业(园林专业)川教函[2019]31号。

摘　　要：为研究多年生黑麦草(Lolium perenne)种子萌发过程中水分代谢规律及水分胁迫对萌发过程生理调控的影响,连续观测黑麦草种子48 h内吸水情况,设置干旱和淹水胁迫处理,研究水分胁迫对黑麦草种子萌发、淀粉代谢、无氧呼吸酶活性以及抗氧化酶活性的影响。结果表明,黑麦草种子0~24 h为快速吸水期,吸水36 h后趋于饱和。干旱处理显著影响了种子的吸水量和吸水率(P<0.05),并随着干旱程度的增加而逐渐降低。15%PEG和淹水胁迫与对照相比都显著降低了黑麦草种子的发芽势、发芽率、发芽指数、α-淀粉酶活性(P<0.05)。淹水胁迫下黑麦草种子的乙醇脱氢酶(ADH)活性显著升高(P<0.05)。干旱和淹水胁迫下黑麦草种子的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性与对照相比均显著增高(P<0.05)。本研究结果为黑麦草种子萌发过程的水分管理提供了理论参考依据。This study aimed to determine the characteristics of water metabolism during seed germination of perennial ryegrass(Lolium perenne)and the effect of water stress on physiological regulation during seed germination.Water absorption by seeds of perennial ryegrass was continuously observed for 48 h,and the seeds were treated to stress conditions of drought and waterlogging,to study the effects of water stress on germination,starch metabolism,anaerobic respiration enzyme activity,and antioxidant enzyme activity in ryegrass seeds.The results showed that ryegrass seeds are in the period of rapid water absorption from 0 to 24 h and tend to saturation after 36 h.Water absorption and water absorption rate of seeds were significantly affected by drought stress(P<0.05),and gradually decreased with the increase in drought degree.Compared with the control,treatments of 15%polyethylene glycol and waterlogging stress significantly reduced the germination energy,germination rate,germination index,andα-amylase activity of ryegrass seeds(P<0.05).Underwaterlogging stress,the alcohol dehydrogenase activity of ryegrass seeds was significantly increased(P<0.05).Compared with the control,under drought and waterlogging stress,the activities of superoxide dismutase,peroxidase,and catalase in ryegrass seeds were significantly increased(P<0.05).The results of this study provide a theoretical reference for water management of the ryegrass seed germination process.

关 键 词：黑麦草 种子萌发 水分胁迫 发芽力 吸水代谢 生理调控 抗氧化酶 

分 类 号：S688.4[农业科学—观赏园艺]