神经病理性痛诱发小鼠睡眠障碍时丘脑-皮质谷氨酸与神经元活动的关系  

Relationship between thalamocortical glutamate and neuronal activity in mice with sleep disorders induced by neuropathic pain

作　　者：李婷婷 钟琦[1] 曹玥 陈婷[1] 王杰 陈畅[1] 张宗泽[1] Li Tingting;Zhong Qi;Cao Yue;Chen Ting;Wang Jie;Chen Chang;Zhang Zongze(Department of Anesthesiology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China;Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院麻醉科,武汉430071 [2]武汉物理与数学研究所精密测量科学与技术创新研究院,武汉430071

出　　处：《中华麻醉学杂志》2022年第5期576-580,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金(81771160,81671060)。

摘　　要：目的评价神经病理性痛诱发小鼠睡眠障碍时谷氨酸与丘脑-皮质神经元活动的关系。方法SPF级健康雄性C57BL/6小鼠28只,6~8周龄,体重15~25 g,采用随机数字表法分为2组(n=14):假手术组(Sham组)和神经病理性痛组(CCI组)。采用结扎坐骨神经干法建立小鼠神经病理性痛模型。于造模前1 d(T_(0))和造模后3、5、7、14、21 d(T_(1~5))时测定术侧足机械缩足反应阈和热缩足潜伏期。T_(3)时植入EEG记录电极到视觉皮层,T_(4)时监测6 h EEG的变化,计算非快动眼睡眠时间、快动眼睡眠时间和觉醒时间占总时间的百分比。T_(3)时利用脑立体定位仪将微丝电极植入到丘脑腹后核(VP)和初级体感皮层(S1),T_(4)时采集VP和S1场电位,计算各波功率百分比,同时评价VP和S1局部场电位的相干性。T_(4)时处死小鼠,取脑组织,采用质子核磁共振波谱检测丘脑和皮质各脑区神经递质水平。结果与Sham组相比,CCI组T_(1~5)时机械缩足反应阈降低,热缩足潜伏期缩短,非快动眼睡眠时间百分比降低,觉醒时间百分比升高,VP区δ波功率百分比降低,VP和S1区α波功率百分比升高,VP-S1场电位的相干性在δ波(1~4 Hz)及α波(8~14 Hz)的频率范围内均增加,丘脑和皮质谷氨酸、谷氨酰胺及谷氨酸-谷氨酰胺水平升高(P<0.05)。结论神经病理性痛诱发小鼠睡眠障碍可能与丘脑-皮质谷氨酸水平升高,导致神经元电活动改变有关。Objective To evaluate the relationship between thalamocortical glutamate and neuronal activity in mice with neuropathic pain-induced sleep disorders.Methods SPF healthy male C57BL/6 mice,aged 6-8 weeks,weighing 15-25 g,were divided into 2 groups(n=14 each)using a random number table method:sham operation group(Sham group)and neuropathic pain group(CCI group).Neuropathic pain was induced by chronic constrictive injury(CCI).The animals were anesthetized with intraperitoneal 10%chloral hydrate 3 ml/kg.The right sciatic nerve was exposed and 4 ligatures were placed on the sciatic nerve at 1 mm intervals.The mechanical paw withdrawal threshold and thermal paw withdrawal latency on the operated side were measured at 1 day before CCI(T_(0))and 3,5,7,14 and 21 days after CCI(T_(1-5)).Electroencephalogram recording electrodes were stereotaxically implanted in visual cortex at T_(3),and electroencephalogram were monitored for 6 h,the percentages of non-rapid eye movement,rapid eye movement and wakefulness in the total time were calculated.Microwire electrodes were implanted epidurally over the ventral posterior(VP)nucleus of the thalamus and primary somatosensory cortex(S1)using a brain stereotaxic apparatus at T_(3),and the data acquisition system was used to record field potentials at T_(4),the percentage of power of each wave was calculated,and the coherence of the field potentials of VP and S1 was simultaneously evaluated.The mice were sacrificed at T_(4),brain tissues were collected,and proton nuclear magnetic resonance spectroscopy was used to determine the level of neurotransmitter in the thalamus and cortex.Results Compared with group Sham,the mechanical paw withdrawal threshold was significantly decreased and thermal paw withdrawal latency was shortened at T_(1-5),the percentage of non-rapid eye movement time was decreased,the percentage of wakefulness time was increased,the percentage ofδwave power in the VP area was decreased,the percentage ofδwave power in the VP and S1 areas was increased,and the coherence o

关 键 词：神经痛 睡眠觉醒障碍 谷氨酸 丘脑 大脑皮质 神经元 

分 类 号：R614[医药卫生—麻醉学]