朱顶红试管苗离体保存及恢复生长研究  

Study on in vitro Conservation and Recovery Growth of Hippeastrum Tube Seedlings

作　　者：陈敏敏[1] 张永春[1] 蔡友铭[1] 苏静 李心[1] 周琳[1] 杨柳燕[1] CHEN Min-min;ZHANG Yong-chun;CAI You-ming;Su Jing;LI Xin;ZHOU Lin;YANG Liu-yan(Forestry and Pomology Research Institute,Shanghai Academy of Agricultural Sciences/Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201403,China)

机构地区：[1]上海市农业科学院林木果树研究所/上海市设施园艺技术重点实验室,上海201403

出　　处：《江西农业学报》2022年第5期69-75,共7页Acta Agriculturae Jiangxi

基　　金：上海市科技创新行动计划“崇明后花博花卉产业发展适用花卉品种研究”(20dz1203900);上海市科学技术委员会“植物种苗组培专业技术服务平台”(21DZ2292300)。

摘　　要：为延长朱顶红离体保存时间、提高存活率,研究了甘露醇、蔗糖、矮壮素、脱落酸对朱顶红试管苗离体保存及恢复生长的影响。结果表明:在培养室温度(15±2)℃、光照强度2500~3000 lx、光照时间10 h/d的条件下,MS培养基中添加20~40 g/L甘露醇能有效抑制朱顶红植株生长,保存5个月后恢复培养2个月的死亡率为0%~40%;75 g/L蔗糖能抑制朱顶红生长发育,离体保存5个月、恢复生长2个月后存活率达100%;0.5~4.0 mg/L ABA能明显抑制朱顶红植株生长,但恢复生长培养后死亡率较其他几种添加物高,恢复生长培养2个月后死亡率最高达50%。10~50 mg/L矮壮素的添加对朱顶红生长抑制效果较差。In order to prolong the in vitro preservation time and improve the survival rate,the effects of mannitol,sucrose,chlormequat chloride and abscisic acid on the in vitro preservation and recovery growth of Hippeastrum tube seedlings were studied.The results showed that the addition of 20~40 g/L mannitol could effectively inhibit the growth of Hippeastrum at(15±2)℃ under the 10 h photoperiod culture condition of 2500~3000 lx,and the mortality rate was 0%~40% after 6 months in vitro storage and 2 months recovery growth.The 75 g/L sucrose could inhibit the growth and development of Hippeastrum,and the survival rate was 100% after 5 months in vitro storage and 2 months recovery growth.The 0.5~4 mg/L ABA could significantly inhibit the growth of Hippeastrum,but the mortality rate was higher than other osmotic regulatory substances.The mortality rate was up to 50% after 5 months in vitro storage and 2 months recovery growth.The 10~50 mg/L chlormequat chloride had a poor inhibition effect on plant growth.

关 键 词：朱顶红 离体保存 蔗糖 甘露醇 矮壮素 脱落酸 

分 类 号：S682.25[农业科学—观赏园艺]