HPLC-DAD法测定益气聪明丸中蔓荆子黄素、异阿魏酸、葛根素和芍药苷  

Determination of vitexicarpin,ferulic acid,puerarin,and paeoniflorin in Yiqi Congming Pills by HPLC-DAD

作　　者：鲍明敏 元晓敏 刘会艳 BAO Ming-min;YUAN Xiao-min;LIU Hui-yan(Department of Chemistry,Anyang Food and Drug Inspection and Testing Center,Anyang 455000,China;Department of Pharmacy,Anyang Third People's Hospital,Anyang 455000,China;Department of Drug Inspection,Hebi Food and Drug Inspection and Testing Center,Hebi 456000,China)

机构地区：[1]安阳市食品药品检验检测中心化学室,河南安阳455000 [2]安阳市第三人民医院药剂科,河南安阳455000 [3]鹤壁市食品药品检验检测中心药品检验科,河南鹤壁456000

出　　处：《现代药物与临床》2022年第6期1244-1247,共4页Drugs & Clinic

摘　　要：目的建立HPLC-DAD法同时测定益气聪明丸中蔓荆子黄素、异阿魏酸、葛根素和芍药苷。方法Waters X-bridge C18色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈–0.5%磷酸溶液,梯度洗脱;检测波长:257 nm(蔓荆子黄素)、315nm(异阿魏酸)、251 nm(葛根素)、230 nm(芍药苷);体积流量:1.0 mL/min;柱温:25℃;进样体积:10μL。结果异阿魏酸、芍药苷、葛根素和蔓荆子黄素分别在0.098~9.830、0.199~19.900、0.239~23.900、0.237~23.700μg/mL线性良好,平均回收率分别为99.57%、99.64%、99.13%、99.64%,RSD值分别为1.79%、1.35%、0.67%、1.43%。结论建立的方法简便快捷、准确可靠,可用于益气聪明丸的质量控制。Objective To establish an HPLC-DAD method for simultaneous determination of vitexicarpin,ferulic acid,puerarin,and paeoniflorin in Yiqi Congming Pills.Methods HPLC analysis was performed on Waters X-bridge C18 column(250 mm×4.6 mm,5μm)with acetonitrile-0.5%phosphoric acid solution by gradient elution.The detection wavelength was set at 257 nm(vitexicarpin),315 nm(ferulic acid),251 nm(puerarin),and 230 nm(paeoniflorin).The flow rate was 1.0 mL/min,the column temperature was 25℃,and the sample volume was 10μL.Results Vitexicarpin,ferulic acid,puerarin,and paeoniflorin showed good linear relationships in concentration range 0.098—9.830,0.199—19.900,0.239—23.900,and 0.237—23.700μg/mL,respectively.The recoveries were 99.57%,99.64%,99.13%,and 99.64%with values of 1.79%,1.35%,0.67%,and 1.43%,respectively.Conclusion The method is simple,fast,accurate,and reliable,and can be applied to the quality control of Yiqi Congming Pills.

关 键 词：益气聪明丸 蔓荆子黄素 异阿魏酸 葛根素 芍药苷 HPLC-DAD 

分 类 号：R286.02[医药卫生—中药学]