FAM20C在小鼠下颌髁突发育过程中的时空表达  

Spatial and temporal expression of FAM20C in mandibular condyle development of mice

作　　者：祁皓 马肃[1,2] 袁朝阳 吴国栋 刘培红[1,2] QI Hao;MA Su;YUAN Zhaoyang;WU Guodong;LIU Peihong(Department of Periodontics,The First Affiliated Hospital of Harbin Medical University,School of Stomatology,Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院·哈尔滨医科大学口腔医学院牙周科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院龙江学者实验室,黑龙江哈尔滨150001 [3]黑龙江省心血管病声光电磁诊疗重点实验室,黑龙江哈尔滨150001

出　　处：《口腔医学》2022年第7期600-603,共4页Stomatology

基　　金：黑龙江省自然科学基金面上项目(H2017030);哈尔滨医科大学附属第一医院科研基金(2016B020)。

摘　　要：目的观察小鼠髁突发育过程的不同阶段,FAM20C在髁突软骨中的时空表达特点,试探究其在小鼠髁突发育过程中的可能作用机制。方法苏木精-伊红(HE)染色和改良番红O-固绿染色观察胚胎17.5 d和出生后0、7、21 d 4组小鼠髁突软骨及软骨下骨的形态学变化。免疫组化染色观察相应时间点FAM20C在小鼠髁突软骨组织中的定位及表达。测量FAM20C阳性表达的平均光密度值,并进行单因素方差分析。结果HE染色和改良番红O-固绿染色结果表明:随着软骨内骨化的进展,髁突软骨细胞层长度减少,软骨下骨体积增加,下颌髁突体积增大;免疫组化结果表明:4组小鼠髁突软骨组织中均有FAM20C阳性表达,FAM20C主要表达于髁突软骨增殖软骨细胞层和前肥大软骨细胞层,少量表达于肥大软骨细胞层及软骨下骨层,随着髁突发育,FAM20C表达逐渐减少。统计学结果显示,各时间点FAM20C阳性表达差异有统计学意义(P<0.01)。结论FAM20C参与小鼠下颌髁突发育,可能通过调节髁突软骨细胞的增殖和分化在髁突形成中发挥重要作用。Objective To observe the spatial and temporal expression of FAM20 C in condylar cartilage at different stages of condylar development in mice and explore its possible mechanism.Methods HE staining and modified safranin O-fast green staining were used to observe morphological changes of condylar cartilage and subchondral bone in 17.5 days of embryo and 0,7,and 21 days after birth.Immunohistochemical staining was used to observe the localization and expression of FAM20 C in mouse condylar cartilage tissues at corresponding time points.The mean optical density values of FAM20 C positive expression were measured and subjected to one-way ANOVA.Results Results of HE staining and modified safranin O-fast green staining showed that with the progression of endochondral ossification,the length of condylar chondrocyte layer decreased;the volume of subchondral bone increased,and the volume of mandibular condyle increased.The immunohistochemical results showed that FAM20 C was positively expressed in condylar cartilage tissue of the four groups of mice.FAM20 C was mainly expressed in the proliferating chondrocyte layer,and pre-hypertrophic chondrocyte layer of condylar cartilage and a small amount was expressed in the hypertrophic chondrocyte layer and subchondral bone layer.With the development of condyle,the expression of FAM20 C gradually decreased.Results of statistical analysis showed that there was a significant difference in FAM20 C positive expression at each time point(P<0.01).Conclusion FAM20 C is involved in mandibular condyle development in mice and may play an important role in condyle formation by regulating the proliferation and differentiation of condylar chondrocytes.

关 键 词：FAM20C 髁突软骨 发育 时空表达 小鼠 

分 类 号：R321.53[医药卫生—人体解剖和组织胚胎学]