不同同期发情方案对奶牛胚胎移植效果的分析  

Effects of different estrous synchronization schemes on embryo transfer in dairy cows

作　　者：赵增元 苏硕青 李欣 刘玉 朱宏波 张普 余文莉 李树静 ZHAO Zengyuan;SU Shuoqing;LI Xin;LIU Yu;ZHU Hongbo;ZHANG Pu;YU Wenli;LI Shujing(Shijiazhuang Tianquan Elite Dairy Co.,Ltd.,Shijiazhuang 050200,China;Hebei Tianhe Beef Cattle Breeding Co.,Ltd.,Shijiazhuang 050200,China;Hebei Dairy Breeding Engineering Research,Centre 050200,China;Hebei Cattle Industry Technology Research Institute,Shijiazhuang 050200,China)

机构地区：[1]石家庄天泉良种奶牛有限公司,石家庄050200 [2]河北天和肉牛养殖有限公司,石家庄050200 [3]河北省奶牛良种繁育工程技术研究中心,石家庄050200 [4]河北省牛产业技术研究院,石家庄050200

出　　处：《黑龙江畜牧兽医》2022年第13期7-13,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：“十三五”国家重点研发计划项目(2017FYD0501905);河北省现代农业产业技术体系奶牛产业创新团队项目(HBCT2018120204);石家庄市科学技术研究与发展计划项目(201500452A);石家庄市科学技术研究与发展计划项目(181500322A);石家庄市鹿泉区县域科技创新跃升项目。

摘　　要：为了提高奶牛胚胎移植效率,试验研究了黄体酮阴道缓释剂+前列腺素(CIDR+PG_(7-10)组、CIDR+PG_(8-10)组、CIDR+PG_(9-10)组、CIDR+PG_(7-8)组、CIDR+PG_(7-9)组、CIDR+PG_(7-10)组)、Ovsynch(079B/G组)和D-Ovsynch(D-079B/G组)3种同期发情-胚胎移植方案对196头育成牛和376头经产牛同期发情的效果和移植妊娠率的影响,并分析了促性腺激素释放激素(GnRH)和布舍瑞林对胚胎移植的效果。根据采用方案不同分为CIDR+PG_(7-10)组、079B/G组、D-079B/G组。结果表明:3种周期发情方案中经产牛发情时间均滞后于育成牛,CIDR+PG_(7-10)组育成牛(100%)和经产牛(94.44%)的发情集中度均好于其他组,且育成牛好于经产牛。各组育成牛和经产牛的移植利用率和妊娠率均差异不显著(P>0.05),但CIDR+PG_(7-10)组移植妊娠率(48.39%和56.67%)均高于其他组,079B组育成牛(93.55%)和D-079B组经产牛(92.86%)移植利用率高于其他组。育成牛冲胚后第7天同期发情,079B组(95.83%)移植利用率高于CIDR+PG_(7-10)组(76.00%)和079G组(72.00%),且差异显著(P<0.05),3组间移植妊娠率差异不显著(P>0.05);随机时间处理时,3组间移植利用率和移植妊娠率均差异不显著(P>0.05);冲胚后第7天与随机时间处理间各组移植利用率和移植妊娠率均差异不显著(P>0.05)。说明同期发情方案CIDR+PG7-10、079G和079B均适用于育成牛胚胎移植,同期发情方案CIDR+PG7-10、D-079G和D-079B均适用于经产牛胚胎移植,GnRH与布舍瑞林的作用效果差异不显著,产后奶牛首配实施CIDR+PG7-10或D-079同期发情-胚胎移植方案是可行的。In order to improve the efficiency of cow embryo transfer,three estrous synchronization schemes of progesterone vaginal sustained re-lease agent+prostaglandin(CIDR+PG_(7-10),CIDR+PG_(8-10),CIDR+PG_(9-10),CIDR+PG_(7-8),CIDR+PG_(7-9),CIDR+PG_(7-10) groups),ovsync(079B/G groups)and D-ovsync(D-079B/G groups)were used to study the effect of estrus synchronization and transfer pregnancy rate on 196 adult cattle and 376 postpartum cattle.The effect of gonadotropin releasing hormone(GnRH)and buserelin on embryo transfer were ana-lyzed,which were divided into CIDR+PG_(7-10),079B/G and D-079B/G groups according to different schemes.The results showed that the es-trus time of parturient cattle in three kinds of cycle estrus programs was lagged behind that of mature cattle.The estrus concentration of adult cattle(100%)and postpartum cattle(94.45%)CIDR+PG_(7-10) group was better than that of other groups,and that of adult cattle was better than that of postpartum cattle.There was no significant difference in the transplantation utilization and pregnancy rate between the adult cattle and the postpartum cattle groups(P>0.05),but the transplantation pregnancy rate(48.39%and 56.67%)of CIDR+PG_(7-10) group was higher than that of other groups,and the transplantation utilization rate of the adult cattle group 079B(93.55%)and the postpartum cattle group D-079B(92.86%)were higher than that of other groups.The utilization rate of transplantation in 079B group(95.83%)was signifi-cantly higher than that of CIDR+PG_(7-10) group(76.00%)and 079G group(72.00%)(P<0.05),but there was no significant difference in transplantation utilization rate and transplantation pregnancy rate among the three groups(P>0.05).There was no significant difference in transplantation utilization and pregnancy rate between 7 days after embryo flushing and random time treatment(P>0.05).These results indica-ted that the estrous synchronization schemes of CIDR+PG_(7-10),079G and 079B were applicable to embryo transfer of adult cattle,and the es-trous synchronization

关 键 词：奶牛 同期发情 胚胎移植 妊娠率 促性腺激素释放激素 布舍瑞林 

分 类 号：S823[农业科学—畜牧学] S814.6[农业科学—畜牧兽医]