流感病毒生命周期中关键RNA结合蛋白的筛选和初步研究  

Screening and Preliminary Study of Crucial RNA⁃binding Proteins in the Life Cycle of Influenza Virus Infection

作　　者：余树芳 蒋智民 魏凡华[1] YU Shufang;JIANG Zhimin;WEI Fanhua(College of Agriculture,Ningxia University,Yinchuan 750021,China;College of Veterinary Medicine,China Agricultural University,Beijing 100094,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]中国农业大学动物医学院,北京100094

出　　处：《病毒学报》2022年第4期807-813,共7页Chinese Journal of Virology

基　　金：国家自然科学基金项目(项目号:31972669),题目:A型流感病毒通过上调PGRN逃逸宿主免疫的分子机制;国家自然科学基金项目(项目号:81960297),题目:DNA受体IFI16在A型流感病毒感染中的作用和机制研究。

摘　　要：流感病毒是一种有囊膜结构、分节段的负链RNA病毒,其复制需要利用宿主的转录翻译系统进行,所以宿主蛋白在流感病毒维持其生命周期及致病性中具有非常重要的作用。为此,通过RNA pull⁃down结合质谱分析筛选了流感病毒生命周期中的关键RNA结合蛋白(RBP)。结果发现了69个可以与流感病毒3个以上病毒RNA(vRNA)片段相互作用的RBP,其中DHX9、IFI27、RBBP6以及SRSF5蛋白显著调控流感病毒的复制。总之,本研究筛选到的RBP为之后进一步解析这些蛋白在调控流感病毒感染周期中的作用机制提供了研究基础。Influenza virus is a kind of encapsulated,segmental,negative strand RNA virus,its replication needs to use the transcription and translation system of the host,thus the host proteins play a very important role in maintaining the life cycle and pathogenicity of influenza virus.To do this,RNA pull⁃down and mass spectrometry were used to screen the key RNA binding proteins(RBPs)in the life cycle of influenza virus.The results demonstrated that 69 RBPs can interact with more than three viral RNA(vRNA)fragments of influenza virus,and confirmed that four genes show a significant changes,including DHX9,IFI27,RBBP6,and SRSF5.In conclusion,the RBPs screened in this study provide a research basis for further analysis of the mechanism of these proteins in the regulation of influenza virus infection cycle.

关 键 词：流感病毒 RNA结合蛋白 Pull⁃down 病毒RNA 病毒与宿主相互作用 

分 类 号：S852.65[农业科学—基础兽医学] R373.1[农业科学—兽医学]