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作 者:李玲 璩骁 张艳宾 张彤 李欣 李鹏宇 肖东 崔水保 齐鹏 肖进 LI Ling;QU Xiao;ZHANG Yanbin;ZHANG Tong;LI Xin;LI Pengyu;XIAO Dong;CUI Shuibao;QI Peng;XIAO Jin(Key Laboratory of Veterinary Bioproduction and Chemical Medicine of the Ministry of Agriculture,Engineering and Technology Research Center for Beijing Veterinary Peptide Vaccine Design and Preparation,Zhongmu Institutes of China Animal Husbandry Industry Co.Ltd,Beijing 100095,China;CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Cahic Jiangxi Biological Pharmaceutical Factory,Nanchang 330200,China)
机构地区:[1]中牧实业股份有限公司农业部兽用生物制品与化药重点实验室,北京市兽用多肽疫苗设计与制备工程技术中心,北京100095 [2]中国科学院微生物研究所,中国科学院病原微生物与免疫学重点实验室,北京100101 [3]中牧股份江西生物药厂,南昌330200
出 处:《病毒学报》2022年第4期915-927,共13页Chinese Journal of Virology
摘 要:盖他病毒(Getah virus,GETV)是一种蚊媒传播病毒,属于披膜病毒科(Togaviridae)甲病毒属(Alphavirus)成员,其感染引起的疾病对我国动物和公共卫生造成的威胁日益严重。本研究在对从江西省某养殖场收集到的兔子脾脏组织进行检测时,经病毒分离、纯化及鉴定,获得一株能够在PK⁃15细胞稳定增殖的GETV分离株,命名为GETV JXNC。全基因组测序结果分析显示,GETV JXNC与GenBank中现有43株GETV参考毒株核苷酸序列相似性为95.3%~99.6%;遗传进化分析结果显示,GETV JXNC与2016年的一株猪源GETV毒株HNNY⁃1(GenBank登录号:MG865966)亲缘关系最近,处于同一进化分支,二者的核苷酸相似性高达99.6%,非结构蛋白编码氨基酸序列相似性为99.7%,结构蛋白编码氨基酸序列相似性为99.8%。本研究首次从家兔中检测并分离到GETV,对GETV的感染谱、传播机制和公共卫生等相关研究具有重要意义。Getah virus(GETV)is a mosquito-borne virus classified in the Alphavirus genus of the Togaviridae family,the infection by which has become an increasing threat to the health of animals and humans in China. We isolated a GETV strain from the homogenized spleen of rabbits in Jiangxi Province,southern China,in October2018. This isolated and characterized GETV strain was named“JXNC”. Phylogenetic analysis reveals that it is clustered in the group Ⅲ genotype,and shared 95.3%-99.6% identity in nucleotide sequences with all available reference strains. The closest relationship of the obtained JXNC strain was with HNNY-1 isolated from pigs in China 2016,with 99.6% nucleotide identity,99.7% amino-acid identity of the nonstructural polyprotein and99.8% amino-acid identity of the structural polyprotein. This is the first time that determination and description of the GETV originating from rabbits has been done in China.
关 键 词:致细胞病变效应 盖他病毒(GETV) 遗传进化树
分 类 号:S852.65[农业科学—基础兽医学]
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