二烯丙基二硫通过miR-7下调XIAP抑制人胃癌SGC7901细胞增殖与迁移侵袭  

Diallyl disulfide downregulates XIAP through miR-7 to inhibit proliferation,migration and invasion of human gastric cancer SGC7901 cells

作　　者：刘芳 李志敏 曹振华 何慧 苏琦 苏波 LIU Fang;LI Zhi-min;CAO Zhen-hua;HE Hui;SU Qi;SU Bo(Cancer Research Institute,Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial University,Hengyang Medical School,University of South China,Hengyang,Hunan 421001,China;Clinical Anatomy&Reproductive Medicine Application Institute,Hengyang Medical School,University of South China,Hengyang,Hunan 421001,China;Institute of Pharmacy and Pharmacology,Hengyang Medical School,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学衡阳医学院肿瘤研究所、湖南省高校肿瘤细胞与分子病理学重点实验室,湖南衡阳421001 [2]南华大学衡阳医学院应用解剖与生殖医学研究所,湖南衡阳421001 [3]南华大学衡阳医学院药物药理研究所,湖南衡阳421001

出　　处：《中国药理学通报》2022年第8期1164-1169,共6页Chinese Pharmacological Bulletin

基　　金：湖南省自然科学基金资助项目(No.2020JJ4522,2020JJ4529);湖南省高校创新平台开放基金(No.17K081)。

摘　　要：目的探讨二烯丙基二硫(diallyl disulfide,DADS)是否通过miR-7下调X连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP),抑制胃癌SGC7901细胞增殖、迁移和侵袭。方法构建XIAP过表达SGC7901细胞株;qRT-PCR、Western blot检测DADS对过表达细胞XIAP表达的影响;CCK-8和平板克隆形成实验分析DADS对XIAP过表达细胞增殖的影响;划痕和Transwell实验分析DADS对XIAP过表达细胞迁移和侵袭的影响;qRT-PCR检测DADS对miR-7表达的影响;双荧光素酶报告实验、qRT-PCR和Western blot验证miR-7对XIAP靶向负调控作用。结果XIAP过表达增强SGC7901细胞增殖、迁移和侵袭能力;DADS下调XIAP、抑制XIAP过表达细胞增殖、迁移和侵袭;DADS上调miR-7表达;miR-7靶向调控XIAP 3′UTR,过表达miR-7降低XIAP表达,而敲低miR-7则上调XIAP表达。结论DADS通过miR-7下调XIAP抑制SGC7901细胞增殖、迁移和侵袭。Aim To investigate whether diallyl disulfide(DADS)downregulates X-linked inhibitor of apoptosis protein(XIAP)through miR-7,inhibiting the proliferation,migration and invasion of gastric cancer SGC7901 cells.Methods Gastric cancer SGC7901 cell line overexpressing XIAP was established.qRT-PCR and Western blot were used to detect the effect of DADS on XIAP expression in overexpressed cells.CCK-8 and plate clone formation assay were used to analyze the effect of DADS on the proliferation of XIAP overexpressing cells.Wound healing and Transwell experiments were employed to analyze the effects of DADS on the migration and invasion of XIAP overexpressing cells.qRT-PCR was used to detect the effect of DADS on the expression of miR-7.Negative regulation of miR-7 on XIAP was verified by using dual luciferase reporter gene assay,qRT-PCR,and Western blot.Results XIAP overexpression enhanced the proliferation,migration and invasion of SGC7901 cells.DADS downregulated XIAP and inhibited the proliferation,migration and invasion of XIAP overexpressing cells.DADS upregulated miR-7 expression.miR-7 bound and regulated XIAP 3′UTR directly.Overexpression of miR-7 decreased XIAP expression,while knockdown of miR-7 increased XIAP expression.Conclusion DADS downregulates XIAP through miR-7 to inhibit SGC7901 cell proliferation,migration and invasion.

关 键 词：二烯丙基二硫 X连锁凋亡抑制蛋白 miR-7 胃癌 增殖 迁移 侵袭 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R394.2[医药卫生—基础医学] R735.2R916.4R977.6