二维柱切换高效液相色谱法测定美白类化妆品中氢醌和苯酚  

Determination of hydroquinone and phenol in whitening cosmetics by column switching two-dimensional HPLC

作　　者：王珵[1] 王嘉庆 张立雯[1] WANG Cheng;WANG Jia-qing;ZHANG Li-wen(Guangzhou Institute for Drug Control,Guangzhou 510160,China)

机构地区：[1]广州市药品检验所,广州510160

出　　处：《药物分析杂志》2022年第6期1065-1071,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：广州市场监督管理局科技项目(2022kj29)。

摘　　要：目的:建立二维柱切换高效液相色谱法测定化妆品中氢醌、苯酚的分析方法。方法:二维色谱柱均为C_(18)色谱柱(250 mm×4.6 mm,5μm),捕获柱为2条串联的C_(16)色谱柱(20 mm×4.0 mm,5μm),10 mmol·L^(-1)磷酸二氢钾溶液(用磷酸调节pH3.5)-甲醇为流动相,梯度洗脱,流速1.0 mL·min^(-1),柱温35℃,检测波长280 nm,进样量2μL。结果:氢醌和苯酚线性范围分别为5.570~111.5μg·mL^(-1)(r=0.9997)和5.410~108.1μg·mL^(-1)(r=0.9996),方法提取回收率分别为91.9%~92.6%和94.4%~94.7%,精密度试验的RSD分别为3.9%~4.1%和0.9%~2.1%,检测下限分别为0.70μg·g^(-1)和0.69μg·g^(-1)。市场随机抽取40批美白类化妆品样品(包含乳剂、霜剂、水剂等)均单次进样即可快速完成样品分析,无基质干扰。结论:本法测定美白类化妆品中氢醌、苯酚含量的专属性好,方法快速、准确,可用于复杂基质的美白类化妆品中氢醌、苯酚的测定。Objective:To establish a method to determine hydroquinone and phenol in whitening cosmetics by switching column two-dimensional HPLC.Methods:Two C_(18) columns(250 mm×4.6 mm,5μm)and two C_(16) trapping columns(20 mm×4.0 mm,5μm)were used with the mobile phase of 10 mmol·L^(-1) monopotassium phosphate solution(adjusted to pH 3.5 with phosphoric acid)-methanol in a gradient elution program at a flow rate of 1 mL·min^(-1).The column temperature was set at 35℃,the determine wavelength was 280 nm,and injection volume was 2μL.Results:Hydroquinone and phenol showed good linear relationship in the ranges of 5.570-111.5μg·mL^(-1)(r=0.9997)and 5.410-108.1μg·mL^(-1)(r=0.9996).The recoveries were 91.9%-92.6%and 94.4%-94.7%with RSDs of 3.9%-4.1%and 0.9%-2.1%,respectively.Forty batches of commercially available whitening cosmetics(including emulsion,cream,astringent,etc)were analyzed by single measurement without matrix interference.Conclusion:The method has good specificity for the determination of hydroquinone and phenol in whitening cosmetics,which can determine hydroquinone and phenol in whitening cosmetics with complex matrix rapidly and accurately.

关 键 词：氢醌 苯酚 二维高效液相色谱 美白类化妆品 分析方法 

分 类 号：R917[医药卫生—药物分析学]