无标记光学成像技术  

Label-Free Optical Imaging Technology

作　　者：穆书奇 董大山 施可彬[1,2] Mu Shuqi;Dong Dashan;Shi Kebin(State Key Laboratory of Artificial Microstructure and Mesoscopic Physics,School of Physics,Peking University,Beijing 100871,China;Collaborative Innovation Center of Extreme Optics,Shanxi University,Taiyuan 030006,Shanxi,China)

机构地区：[1]北京大学物理学院人工微结构和介观物理国家重点实验室,北京100871 [2]山西大学极端光学协同创新中心,山西太原030006

出　　处：《激光与光电子学进展》2022年第12期1-21,共21页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(92150301,91750203,91850111,12004013);广东省基础与应用基础研究重大项目(2020B0301030009)。

摘　　要：无标记光学成像以非侵入性的特点,能够对生物活体细胞进行长时程、无损伤的高分辨观测,在生物医学和临床诊断上有着巨大的前景。无标记成像技术可以分为特异性成像和非特异性成像两类,主要概述目前常用的无标记成像技术。详细介绍了各种无标记成像技术的成像原理、优缺点和最新研究进展。最后,对无标记光学成像未来的发展进行展望。Label-free optical imaging technology can perform long-term, noninvasive, high-resolution imaging on living cells owing to its noninvasive characteristics. This technology has promising applications in biomedical research and clinical diagnosis. Label-free imaging technologies can be categorized into specific imaging and nonspecific imaging. In this paper, we review the commonly used label-free imaging. Herein, the imaging principles, advantages and disadvantages, as well as the recent progresses of unmarked imaging are introduced in detail. Furthermore, the future development of the label-free imaging technology is prospected.

关 键 词：无标记显微成像 特异性成像 非特异性成像 生物光子学 

分 类 号：O439[机械工程—光学工程]