填料优化反硝化脱硫工艺系统的生物强化效果研究  

PROMOTION OF BIO-AUGMENTATION EFFECT BY DENITRIFYING SULFIDE REMOVAL PROCESS WITH FILLER OPTIMIZED

作　　者：郜爽[1] 李智灵[1] 王爱杰[1] 黄聪[1,2] GAO Shuang;LI Zhiling;WANG Aijie;HUANG Cong(State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China)

机构地区：[1]哈尔滨工业大学水资源与环境国家重点实验室,哈尔滨150090 [2]中国科学院天津工业生物技术研究中心,天津300308

出　　处：《环境工程》2022年第4期29-34,70,共7页Environmental Engineering

基　　金：国家自然科学基金资助项目“有机废水碳氮硫共脱除系统微生物功能网络及调控机制”(51808166)。

摘　　要：在反硝化脱硫工艺体系内投加填料,进一步促进了菌剂的生物强化效果,实现了核心功能菌群的优化调控,提升了反硝化脱硫工艺体系的效能。研究发现:填料系统将单质硫的生成效能提升到对照组的1.5倍左右。无生物强化时填料表面生物膜中优势菌属为Pseudomonas和Azoarcus;填料和菌剂共同作用,可以将单质硫生成效能提升到对照组的2倍,此时生物膜优势菌属为Pseudomonas和Arcobacter,生物强化促进了硫氧化功能基因的表达。投加Pseudomonas sp.gs1进行生物强化,提升了填料表面生物膜的抗冲击负荷能力,系统经过冲击后单质硫生成率可以迅速恢复。The promotion of bio-augmentation by filler in a denitrifying sulfide removal process was realized by adding the bacterial agent to regulate the core functional flora,and the efficiency of the denitrification and desulfurization process were improved.It was revealed that the application of filler increased the efficiency of elemental sulfur generation to about 1.5 times of the control group.The dominant bacteria in the biofilm on the filler surface were Pseudomonas and Azoarcus.The combined effect of filler and bacterial agent increased the efficiency of elemental sulfur generation to twice of the control group,and then the dominant bacteria in the biofilm on the filler after bio-augmentation were Pseudomonas and Arcobacter.Expression of sulfur oxidation functional genes was strengthened by bio-augmentation.The artificially added Pseudomonas sp.gs1 improved the impact load resistance of the biofilm on the surface of the filler,and the elemental sulfur generation rate was quickly restored after the impact.

关 键 词：填料 生物强化 反硝化脱硫工艺 复杂碳源 核心功能菌 

分 类 号：X703[环境科学与工程—环境工程] X172