益气养阴活血通络法对急性心肌梗死大鼠Sirt1-FoxO1-FoxO3a信号通路的影响  

Effect of Tonifying Qi,Nourishing Yin,Promoting Blood Circulation and Dredging Collaterals on Sirt1-FoxO1-FoxO3a Signaling Pathway in Rats with Acute Myocardial Infarction

作　　者：路爽[1] 杨莺[2] LU Shuang;YANG Ying(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2022年第6期123-125,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81603605);辽宁省自然科学基金(2019-ZD-0964)。

摘　　要：目的探讨益气养阴、活血通络法对急性心肌梗死大鼠Sirt1-FoxO1-FoxO3a信号通路的影响。方法随机将60只大鼠分组,共6组,依次为空白组,模型组,中药双参活血颗粒高、中、低剂量组和西药(盐酸曲美他嗪)组,将大鼠冠状动脉左前降支结扎后制成急性心肌梗死模型,模型制备成功后连续给药2周,随后处死,采用Western Blot、RT-PCR检测模型大鼠缺血心肌组织中Sirt1、FoxO1、FoxO3a各个蛋白含量与其mRNA的表达。结果模型组中的Sirt1、FoxO1、FoxO3a蛋白含量、mRNA表达与空白组比均下降(P<0.05),中药各剂量组与西药组Sirt1、FoxO1、FoxO3a蛋白含量、mR-NA表达与模型组比均上升(P<0.05),中药组上升幅度具有药物浓度依赖性,西药组与中药高剂量组比较差异无统计学意义(P>0.05)。结论具有益气养阴、活血通络功效的中药双参活血颗粒可以影响Sirt1-FoxO1-FoxO3a信号通路而减轻大鼠急性心肌梗死的损伤。Objective To investigate the effects of tonifying qi and nourishing Yin,promoting blood circulation and clearing col-laterals method on sirtuin1(Sirt1)-forkhead box protein O1(FoxO1)-forkhead box O3a(FoxO3a)signaling pathway in rats with acute myocardial infarction.Methods Sixty rats were randomly divided into blank group,model group,Shuangshen Huoxue Granule(双参活血颗粒)high,medium and low dose groups and trimezidine hydrochloride group.The acute myocardial infarc-tion model was made by ligation of the left anterior descending coronary artery in rats.After the model was successfully prepared,the rats were given continuous medication for 2 weeks,and then they were sacrificed.The protein contents of Sirt1,FoxO1 and FoxO3a and their mRNA expressions in ischemic myocardium of model rats were detected by Western Blot and RT-PCR.Re-sults The protein contents and mRNA expressions of Sirt1,FoxO1 and FoxO3a in the model group were decreased compared with those in the blank group(P<0.05).The protein contents and mRNA expressions of Sirt1,FoxO1 and FoxO3a in the Shuangshen Huoxue Granule groups and the western medicine group were increased compared with those in the model group(P<0.05).There was no significant difference between the western medicine group and the Shuangshen Huoxue Granule high dose group(P>0.05).Conclusion Shuangshen Huoxue Granule with the effects of nourishing Qi,nourishing Yin and activating blood cir-culation can affect Sirt1-FoxO1-FoxO3A signaling pathway and alleviate the injury of acute myocardial infarction in rats.

关 键 词：双参活血颗粒 益气养阴活血通络法 急性心肌梗死 Sirt1-FoxO1-FoxO3a信号通路 

分 类 号：R285.5[医药卫生—中药学]