牛蒡苷元纳米给药体系抗卵巢癌体内外研究  

In Vitro and In Vivo Study on Arctigenin Nano Drug Delivery System against Ovarian Cancer

作　　者：史锐[1,2] 刘苗苗 丛龙娇 刘斯文 黄晓彤 SHI Rui;LIU Miaomiao;CONG Longjiao;LIU Siwen;HUANG Xiaotong(Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications,Ministry of Education of China,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Liaoning University of Traditional Chinese Medicine Liaoning,Shenyang 110032,Liaoning,China)

机构地区：[1]辽宁中医药大学中医脏象理论及应用教育部重点实验室,辽宁沈阳110847 [2]辽宁中医药大学,辽宁沈阳110032

出　　处：《中华中医药学刊》2022年第6期217-220,I0043-I0045,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省自然科学基金计划重点项目(20170540632);辽宁中医药大学中医脏象理论及应用国家教育部重点实验室开放基金(zyzx2001)。

摘　　要：目的通过对卵巢癌SK-OV-3细胞的体外影响及对卵巢癌裸鼠的初步药效研究,考察自制牛蒡苷元FA@ZnO-MSN-ARG给药体系的肿瘤的抑制情况。方法通过采用MTT比色法、流式细胞术检测、卵巢癌小鼠抑瘤率等方法验证FA@ZnO-MSN-ARG给药体系对卵巢癌的抑制作用。结果FA@ZnO-MSN-ARG给药体系的对细胞抑制率明显提升(P<0.05),浓度为25μg/mL时,包装后的牛蒡苷元与包装后的紫杉醇抑制率相差无几;细胞凋亡中,FA@ZnO-MSN-ARG放入药物24 h的细胞凋亡率为12.01%,48 h的细胞凋亡率为18.58%+1.99%,虽低于紫杉醇组,但缓释能力较紫杉醇组高,具有更强的抑制作用;卵巢癌裸鼠抑瘤率测定结果,FA@ZnO-MSN-ARG组和FA@ZnO-MSN-TAXOL组减掉的瘤体大小十分接近,FA@ZnO-MSN纳米给药体系装载下的紫杉醇组和牛蒡苷元组的抑瘤效果明显好于单纯给药组(P<0.05)。结论FA@ZnO-MSN-ARG给药体系抗卵巢癌SK-OV-3细胞活性和肿瘤抑制率与临床常用药物紫杉醇相当,提高了牛蒡苷元的抗肿瘤活性。Objective To investigate the effect of arctigenin on ovarian cancer SK-OV-3 cells in vitro and its preliminary ef-ficacy on ovarian cancer nude mice to observe the tumor inhibition of FA@ZnO-MSN-ARG drug delivery system.Methods The inhibitory effect of FA@ZnO-MSN-ARG drug delivery system on ovarian cancer was verified by MTT colorime-try,flow cytometry and tumor inhibition rate in mice with ovarian cancer.Results The cell inhibition rate of FA@ZnO-MSN-ARG drug delivery system was significantly increased(P<0.05)and the inhibitory rate of the packaged lappaglyin was almost the same as that of the packaged paclitaxel at 25μg/mL.The apoptosis rate was 12.01%after FA@ZnO-MSN-ARG was put into the drug for 24 h,and the apoptosis rate was 18.58%+1.99%after 48 h,which was lower than that of the paclitaxel group,but the sustained release ability was higher than that of the paclitaxel group,and it had stronger inhibitory effect.The results of tumor inhibition rate in nude mice with ovarian cancer showed that the tumor size of the FA@ZnO-MSN-ARG group was very similar to that of the normal group.The tumor inhibition effect of the paclitaxel group and the burdocin group loaded with nano drug delivery system was significantly better than that of the simple drug delivery group(P<0.05).Conclusions The anti-tumor activity and tumor inhibition rate of FA@ZnO-MSN-ARG drug delivery system are similar to that of paclitaxel,which can improve the anti-tumor activity of burdock aglyconin.

关 键 词：卵巢癌 牛蒡苷元 SK-OV-3细胞 裸鼠 

分 类 号：R273.373.1[医药卫生—中西医结合]