碱处理对污泥产甲烷与中间产物氨基酸的影响及两者相关性研究  

EFFECTS OF ALKALI TREATMENT ON METHANE AND INTERMEDIATE AMINO ACIDS AND THEIR CORRELATION DURING ANAEROBIC DIGESTION OF WASTE ACTIVATED SLUDGE

作　　者：徐慧婷 刘辉 马长文[1] 陈浩 朱志浩 徐天陈 叶建锋[5] XU Hui-ting;LIU Hui;MA Chang-wen;CHEN Hao;ZHU Zhi-hao;XU Tian-chen;YE Jian-feng(School of Resources and Environmental Engineering,Shanghai Polytechnic University,Shanghai 201209,China;Shanghai Academy of Environmental Sciences,Shanghai 200233,China;College of Marine Ecology and Environment,Shanghai Ocean University,Shanghai 201306,China;College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China;State Key Laboratory of Pollution Control and Resource Reuse,College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China)

机构地区：[1]上海第二工业大学资源与环境工程学院,上海201209 [2]上海市环境科学研究院,上海200233 [3]上海海洋大学海洋生态与环境学院,上海201306 [4]东华大学环境科学与工程学院,上海201620 [5]同济大学环境科学与工程学院污染控制与资源再利用国家重点实验室,上海200092

出　　处：《环境工程》2022年第5期88-94,共7页Environmental Engineering

基　　金：国家自然科学基金(51908355);上海自然科学基金(19ZR1443700);国家重点研发计划(2017YFE0135500);上海科技创新行动计划(19DZ1204500)

摘　　要：以剩余污泥为底物,研究了碱处理pH对污泥有机物溶出,水解产生的氨基酸种类和构型以及厌氧消化产甲烷的影响。分别采用2种动力学模型对产甲烷过程进行拟合,并利用Spearman分析考察了氨基酸与产甲烷之间相关性。结果显示:碱处理pH为11时COD溶出率最大,最终累积产甲烷量较空白组提高了1.4倍。改进Gompertz模型对产甲烷过程拟合效果较好,拟合系数均在0.989以上,误差范围在3.9%以内。碱处理可提高水解产物氨基酸含量和种类,溶出的氨基酸同时具有L型和D型,其中L、D-Cys含量最高。L型氨基酸含量高于其对映体(Thr除外),Thr的外消旋化程度(D/(D+L))最高(54.0%),其次为Cys(45.5%)和Ala(44.0%)。氨基酸中D-Leu、D-Asp、L-Thr和L-Cys含量与甲烷产量之间有较强的相关性,其Spearman系数分别为0.894、0.9、0.9和0.9(P<0.05)。这为后续研究氨基酸及其构型对厌氧消化产甲烷的影响提供理论参考,为调控厌氧消化提供新的思路。The effects of alkali treatment pH on the dissolution of organic matter,the types and configuration of amino acids produced by proteolysis and methane production by anaerobic digestion of waste activated sludge were studied.Two kinetic models were used to fit the methanogenesis process,and Spearman analysis was used to investigate the correlation between intermediate amino acids and methane production.The results showed that,when alkali treatment pH was 11,the cumulative methane production was 1.4 times higher than that of the control group.The modified Gompertz model had a good fitting effect on the methanogenesis process,the fitting coefficient was above 0.989,and the error range was within 3.9%.Alkali treatment could increase the content and types of amino acids by proteolysis.The dissolved amino acids have L-type and D-type,with the highest content of L-and D-Cys.The content of L-type was higher than its corresponding D-type(except Thr),in which the racemization degree(D/(D+L))of Thr was the highest(54.0%),followed by Cys(45.5%)and Ala(44.0%).Among amino acids,D-Leu,D-Asp,L-Thr and L-Cys had a strong correlation with methane production,and their Spearman coefficients were 0.894,0.9,0.9 and 0.9(P<0.05),respectively.This study provides a theoretical basis for the follow-up study on the effects of amino acids and their configurations on anaerobic digestion and as well as a new idea for the regulation of anaerobic digestion.

关 键 词：碱处理 剩余污泥 氨基酸对映体 产甲烷动力学模型 Spearman相关性 

分 类 号：X703[环境科学与工程—环境工程]