非洲猪瘟病毒污染运输车辆模拟消毒试验  

Experiment on Simulative Disinfection for Vehicles Contaminated by African Swine Fever Virus

作　　者：刘俊 杨作丰 张皓淳 魏澍 陈扬扬 吴晓傲 王宏燕 庞学敏 董娜 刘金玲[3] Liu Jun;Yang Zuofeng;Zhang Haochun;Wei Shu;Chen Yangyang;Wu Xiao'ao;Wang Hongyan;Pang Xuemin;Dong Na;Liu Jinling(Liaoning Agricultural Development and Service Center,Shenyang,Liaoning 110015,China;Kangping Xinwang Agriculture and Animal Husbandry Co.,Ltd.,Shenyang,Liaoning 110500,China;Shenyang Agricultural University,Shenyang,Liaoning 110161,China;Shenyang Agricultural Comprehensive Administrative Law Enforcement Team,Shenyang,Liaoning 110031,China)

机构地区：[1]辽宁省农业发展服务中心,辽宁沈阳110015 [2]康平新望农牧有限公司,辽宁沈阳110500 [3]沈阳农业大学,辽宁沈阳110161 [4]沈阳市农业综合行政执法队,辽宁沈阳110031

出　　处：《中国动物检疫》2022年第8期127-131,共5页China Animal Health Inspection

基　　金：国家自然基金联合基金项目(U20A2060);辽宁省“兴辽英才计划”项目(XLYC1907094)。

摘　　要：为验证碾压消毒垫和驶过消毒池两种常见运输车辆消毒方式对非洲猪瘟病毒(ASFV)的杀灭效果,采用生物学特性和理化特性相近的猪伪狂犬病毒(PRV)疫苗稀释液喷洒运输车辆车轮,测试两种消毒方式的消毒效果。结果显示:车辆碾压消毒垫后0、1、2、3、4、5 min以及驶过消毒池后0、2、5、10 min,车轮可被检测的PRV与表面细菌含量均呈下降趋势,且杀灭效果随时间增加而增强。其中,碾压消毒垫后5 min无法有效杀灭所有PRV,细菌杀灭率为92.5%;驶过消毒池后10 min可有效杀灭所有PRV,细菌杀灭率为98.3%。本研究结果为提高非洲猪瘟疫情应急处置水平和日常防控消毒措施有效性提供了数据支撑。In order to verify the efficacy of two common disinfection methods for vehicles(including rolling on disinfection pads and driving through disinfection pools)on African swine fever virus(ASFV),the wheels of vehicles were sprayed by vaccine diluent of pseudorabies virus(PRV)with similar biological,physical and chemical characteristics to test the disinfection effects of the two methods.The results showed that the volume of PRV and epiphytic bacteria decreased and the effect increased with time after vehicles rolled on disinfection pads for 0,1,2,3,4 and 5 min,and driving through disinfection pools for 0,2,5 and 10 min.Specifically,all PRV could not be effectively killed in 5 min after rolling on disinfection pads,with the bacterial killing rate of 92.5%;but all PRV could be effectively killed in 10 min after driving through disinfection pools,with the bacterial killing rate of 98.3%.Data support was thus provided by the paper for improving the emergency response to ASF outbreak and the effectiveness of daily prevention,control and disinfection measures.

关 键 词：AFSV 运输车辆 碾压消毒 消毒池消毒 

分 类 号：S851.3[农业科学—预防兽医学]