米诺环素对活性污泥初期增殖阶段的影响  

Impact of minocycline on the initial formation of activated sludge

作　　者：孟德伟 池勇志 田野[2,3] 施雯 杨敏[2,3] 张昱[2,3] 田哲[2,3] MENG Dewei;CHI Yongzhi;TIAN Ye;SHI Wen;YANG Min;ZHANG Yu;TIAN Zhe(School of Environmental and Municipal Engineering,Tianjin Chengjian University,Tianjin 300384,China;State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Science,Beijing 100085,China;National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)

机构地区：[1]天津城建大学,环境与市政工程学院,天津300384 [2]中国科学院生态环境研究中心,环境水质学国家重点实验室,北京100085 [3]中国科学院生态环境研究中心,工业废水无害化与资源化国家工程研究中心,北京100085

出　　处：《环境工程学报》2022年第6期1957-1967,共11页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(51978645,31861143049)。

摘　　要：采用污泥连续培养的方式,分别考察了米诺环素在质量浓度为0、0.001、0.01、0.1、1和10 mg·L^(-1)水平下对活性污泥初期增殖阶段的影响。结果表明,各组污泥增殖均能在20 d内进入平台期,且组间的稳态污泥量差异不显著(P>0.05),表明活性污泥宏观生物量的增长与米诺环素浓度无关,但10 mg·L^(-1)米诺环素的暴露可抑制污泥絮体的形成。细菌群落多样性的分析结果表明,1 mg·L^(-1)的米诺环素即可导致活性污泥初期增殖阶段细菌多样性的降低,10 mg·L^(-1)的米诺环素会选择性富集Flavobacterium菌属,使其丰度在第20天的活性污泥细菌群落中达到83.14%,可进一步降低形成的活性污泥细菌多样性。网络分析结果表明,Proteobacteria细菌是活性污泥初期增殖阶段的关键物种,低质量浓度米诺环素(0.001 mg·L^(-1)和0.01 mg·L^(-1))可能促进了活性污泥细菌间的互作,而高质量浓度(1 mg·L^(-1)和10 mg·L^(-1))暴露会显著降低互作网络的复杂度和稳定性。本研究结果可为含抗生素废水的高效生化处理提供参考。Activated sludge(AS) was continuously incubated at the mass minocycline concentrations of 0,0.001,0.01,0.1,1 and 10 mg·L^(-1),respectively,and the effects of these concentrations on the initial formation of AS were investigated.The results showed that the biomass proliferation in 0~10 mg·L^(-1) minocycline treatments all entered the plateau stage within 20 days,and there were no significant differences(P> 0.05) in the steadystate sludge concentrations among the treatments,indicating that the growth of AS biomass was not delayed by the addition of minocycline,but the exposure of 10 mg·L^(-1) minocycline could lead to the failure of the formation of AS flocs.Miseq sequencing of 16S rRNA gene amplicons showed that the bacterial diversity of AS at its initial proliferation stage decreased in 1 mg·L^(-1) minocycline treatment,and 10 mg·L^(-1) minocycline exposure selectively enriched the genus Flavobacterium,and its abundance could reach 83.14% in the AS bacterial community at the end of the experiment,then the diversity of the formed AS bacterial community was further reduced.Network analysis showed that members of Proteobacteria were the key players in the initial formation of AS.Low mass concentration minocycline(0.001 and 0.01 mg·L^(-1)) might promote the interaction between different modules of AS bacterial communities,while high mass concentration(1 and 10 mg·L^(-1)) exposure would significantly reduce the complexity and stability of AS network.The results of the study can provide a reference for the efficient biochemical treatment of antibiotic-containing wastewater.

关 键 词：米诺环素 活性污泥 增殖 群落构建 网络分析 

分 类 号：X703.1[环境科学与工程—环境工程]