CANON工艺中短程硝化恢复及ANAMMOX强化策略  

Short-cut nitrification recovery and ANAMMOX enhancement strategy in CANON process

作　　者：付昆明 杨帆 杨宗玥 曹秀芹 仇付国 吴青 刘永剑 李飞 FU Kunming;YANG Fan;YANG Zongyue;CAO Xiuqin;QIU Fuguo;WU Qing;LIU Yongjian;LI Fei(School of Environment and Energy Engineering,Key Laboratory of Urban Storm Water System and Water Environment,Beijing University of Civil Engineering and Architecture,Sino-Dutch R&D Center for Future Wastewater Treatment Technologies,Beijing 100044,China;Water Design Group Company Limited,Jinan 250000,China)

机构地区：[1]北京建筑大学环境与能源工程学院,北京建筑大学雨水系统与水环境教育部重点实验室,中-荷污水处理技术研发中心,北京100044 [2]水发规划设计有限公司,济南250000

出　　处：《环境工程学报》2022年第6期1991-2000,共10页Chinese Journal of Environmental Engineering

基　　金：北京建筑大学市属高校基本科研业务费专项(X20136)。

摘　　要：为了对CANON工艺中遭破坏的短程硝化进行恢复,并对ANAMMOX菌的活性进行强化,在第1阶段(抑制期)采用连续流反应器,限制DO质量浓度为0.1~0.4 mg·L^(-1),利用限氧条件对NOB活性进行抑制,投加NH_(4)^(+)-N和NO_(2)^(-)-N,经过73d运行,ANAMMOX菌活性由0.08kg·(kg·d)^(-1)(以VSS计)上升至0.34kg·(kg·d)^(-1);NOB比耗氧速率(SOUR)由1.68 kg·(kg·d)^(-1)(以VSS计)降低至0.79 kg·(kg·d)-1,活性显著降低,系统TN去除率由42.7%升高至88.6%,NH_(4)^(+)-N和NO_(2)^(-)-N同步去除,ΔNO_(3)^(-)-N/ΔTN值向理论值0.127趋近。第2阶段(过渡期)、第3阶段(好氧期)采用SBR进行,分别将DO维持在0.4~0.7 mg·L^(-1)和0.7~1.0 mg·L^(-1),至第130天,NOB活性降低至0.57 kg·(kg·d)^(-1),TN去除负荷达到0.86 kg·(m^(3)·d)^(-1),ΔNO_(3)^(-)-N/ΔTN值由抑制前的0.318降低至0.136,短程硝化基本恢复。在短程硝化恢复过程中,ANAMMOX菌优势菌属Candidatus Kuenenia的相对丰度由7.91%增长至13.12%,但NOB的主要菌属Nitrospira的相对丰度由低于0.01%增至1.03%,表明在后续长期运行过程中,依然存在短程硝化遭到破坏的风险。In order to recover the short-cut nitrification in CANON process and enhance the activity of anaerobic ammonium oxidation(ANAMMOX) bacteria,at the first stage(inhibition period),a continuous flow reactor was used and the dissolved oxygen(DO) concentration was limited to 0.1-0.4 mg·L^(-1),so as to inhibit the activity of NOB by oxygen limitation.At the same time,NH_(4)^(+)-N and NO_(2)^(-)-N was added.After 73 days of operation,the activity of ANAMMOX bacteria increased from 0.08 kg·(kg·d)^(-1)(calculated as VSS) to 0.34kg·(kg·d)^(-1).The specific oxygen uptake rate(SOUR) of nitrite oxidizing bacteria(NOB) decreased from 1.68kg·(kg·d)^(-1)(calculated as VSS) to 0.79 kg·(kg·d)^(-1),indicating a significant decrease in the activity of NOB.The total nitrogen(TN) removal rate of the system increased from 42.7% to 88.6%,NH_(4)^(+)-N and NO_(2)^(-)-N were removed simultaneously,and the ΔNO_(3)^(-)-N/ΔTN value approached to the theoretical value of 0.127.The second stage(transition period) and the third stage(aerobic period) were carried out by SBR mode,and the DO concentration maintained at 0.4~0.7 mg·L^(-1) and 0.7~1.0 mg·L^(-1),respectively.On the 130th day,the activity of NOB decreased to 0.57 kg·(kg·d)^(-1),and the TN removal load reached 0.86 kg·(m3·d)^(-1).Meanwhile,the ΔNO_(3)^(-)-N/ΔTN value decreased from 0.318 before the inhibition to 0.136,and the short-cut nitrification was basically recovered.In the process of short-cut nitrification recovery,the relative abundance of the dominant ANAMMOX bacteria,Candidatus Kuenenia,increased from 7.91% to 13.12%,while the relative abundance of the main NOB bacteria,Nitrospira,increased from less than 0.01% to 1.03%,this indicated that a risk of shortcut nitrification destruction will still exist in the following long-term operation.

关 键 词：全程自养脱氮 短程硝化 厌氧氨氧化 亚硝酸盐氧化菌 溶解氧 

分 类 号：X703.1[环境科学与工程—环境工程]