有机硒化合物乙烷硒啉抑制腺样囊性癌细胞生长的体外研究  

Inhibition of adenoid cystic cancer cell growth by ethaselen in vitro

作　　者：邢凤霞 张强[1] 卢国徽 赵艳芳[1] 周琼 XING Fengxia;ZHANG Qiang;LU Guohui;ZHAO Yanfang;ZHOU Qiong(Dental Department,Beifang District of Peking University Third Hospital,Beijing 100089,China)

机构地区：[1]北京大学第三医院北方院区口腔科,北京100089

出　　处：《大医生》2022年第14期1-4,共4页Doctor

摘　　要：目的探讨乙烷硒啉(BBSKE)对腺样囊性癌细胞的抑制作用及机制,为相关研究提供参考。方法用3(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测BBSKE对腺样囊性癌细胞系(SACC-83、SACC-LM)的敏感性。BBSKE作用SACC 48 h后,用赫斯特(Hoechst)荧光染色法检测细胞凋亡形态;BBSKE作用SACC 48 h后,检测BBSKE对腺样囊性癌细胞半胱氨蛋白水解酶-3(Caspase-3)活性的影响。用[5,5-二硫双(2-硝基苯甲酸)](DTNB)法检测BBSKE对腺样囊性癌细胞中硫氧还蛋白还原酶(TrxR)活性的影响。结果BBSKE可以明显抑制腺样囊性癌细胞的增殖,作用于SACC-83和SACC-LM腺样囊性癌细胞72 h,抑制肿瘤细胞生长达到50%时的药物浓度(IC50)分别为12μmol/L和10μmol/L。BBSKE作用SACC 48 h后,细胞发生核固缩、核破碎等形态变化;随着BBSKE浓度增大,SACC-83细胞中Caspase-3活性显著增高,SACC-LM细胞中Caspase-3活性增加更明显;TrxR活性随BBSKE浓度增大而降低。结论新型含硒化合物BBSKE可以抑制腺样囊性癌细胞的增殖。其作用可能是通过抑制TrxR活性,增加半胱氨蛋白水解酶-3活性而实现的。Objective To investigate the inhibitory effect and mechanism of ethaselen(BBSKE)on adenoid cystic cancer cells,and to provide reference for related research.Methods The sensitivity of BBSKE to adenoid cystic carcinoma cell lines(SACC-83,SACC-LM)was determined by methyl thiazolyl tetrazolium(MTT)assay.The apoptotic morphology of SACC cells was detected by Hoechst fluorescence staining after BBSKE treatment for 48 h.The effect of BBSKE on caspase-3 activity of adenoid cystic carcinoma cells were detected after 48 h of SACC treatment.The effect of BBSKE on the activity of thioredoxin reductase(TrxR)in adenoid cystic carcinoma cells were determined by 5,5-dithioubisulfide(2-nitrobenzoic acid)(DTNB)assay.Results BBSKE could significantly inhibit the proliferation of adenoid cystic carcinoma cells,and inhibited the growth of adenoid cystic carcinoma cells of SACC-83 and SACC-LM for 72 h with IC50 of 12μmol/L and 10μmol/L,respectively,when the growth of tumor cells reached 50%.After BBSKE treatment for 48 h,the SACC cells underwent morphological changes such as nuclear pyknosis and nuclear fragmentation.With the increase of BBSKE concentration,caspase-3 activity in SACC-83 cells increased significantly,and caspase-3 activity in SACC-LM cells increased more significantly.TrxR activity decreased with the increase of BBSKE concentration.Conclusion A novel selenium-containing compound BBSKE can inhibit the proliferation of adenoid cystic cancer cells.The effect may be achieved by inhibiting TrxR activity and increasing cysteine hydrolase-3 activity.

关 键 词：乙烷硒啉 腺样囊性癌 硫氧还蛋白还原酶 

分 类 号：R331[医药卫生—人体生理学]