镧系配合物固载亚甲基蓝为信号标签及3D DNA步行器构建传感器用于microRNA-141检测  

Novel biosensor for microRNA-141 analysis based on lanthanide coordination polymer immobilized methylene blue as a signal label and 3D DNA walking machine

作　　者：文燕 邹晓川[3] 王存 钟玲 WEN Y an;ZOU Xiaochuan;WANG Cun;ZHONG Ling(Department of Nephrology,The Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China;Department of Biological and Chemical Engineering,Chongqing University of Education,Chongqing 400067,China;Department of Nephrology,Chongqing Ninth People's Hospital,Chongqing 400700,China)

机构地区：[1]重庆医科大学附属第二医院肾内科,重庆400010 [2]重庆市第九人民医院肾内科,重庆400067 [3]重庆第二师范学院生物与化学工程学院,重庆400700

出　　处：《分析试验室》2022年第7期826-833,共8页Chinese Journal of Analysis Laboratory

摘　　要：MicroRNA-141作为重要的肿瘤标志物,其灵敏检测对癌症的早期诊断和预后评估具有重要意义。基于镧系配合物固载亚甲基蓝为信号标签及三维(3D)DNA步行器信号放大策略,建立了一种microRNA-141生物传感器。首先合成稻草状镧系配合物(Ce-COP)。该Ce-COP具有大的比表面积,可以固载大量的纳米金(AuNPs)和亚甲基蓝(MB)信号分子。其次设计3D DNA步行器,通过机器的高效运转,可将痕量目标microRNA-141转化为大量二级目标物,显著放大传感器的信号。随着目标物浓度增加,传感器电化学信号逐渐增大,microRNA-141在100 amol/L~100 nmol/L范围内呈良好的线性关系,检出限为33 amol/L。该传感器表现出好的稳定性和选择性,健康人血清样品中microRNA-141的加标回收实验结果符合要求。该方法为各种癌症标志物的高灵敏检测提供了一定的参考和借鉴。MicroRNA-141 is of great significance in the early diagnosis and treatment of related cancers.A biosensor was developed for the detection of microRNA-141 based on lanthanide coordination polymer immobilized methylene blue as a signal label and three-dimensional(3D)DNA walking machine.First,lanthanide coordination polymer(Ce-COP)with straw-sheaf like structure was successfully prepared with a large scale via a simple,mild and rapid solution phase method at room temperature.The Ce-COP had a large specific surface area and can immobilize a large number of gold nanoparticles and methylene blue(MB)signal molecules.Then,a 3D DNA walking machine was designed and introduced to the biosensor system for converting trace target microRNA-141 to a larger number of intermediate DNA and achieving significant signal amplification.The sensing platform exhibited recommendable stability and good selectivity.MicroRNA-141 could be accurately quantified with a linear range of 100 amol/L-100 nmol/L with the detection limit of 33 amol/L.This method provides a certain reference to high-sensitive detection for various cancer markers.

关 键 词：镧系配合物 亚甲基蓝 3D DNA步行器 microRNA-141 生物传感器 

分 类 号：O657.1[理学—分析化学] R73.34[理学—化学]