不同冷冻保护剂在血管深低温保存中的研究进展  

Research Progress of Different Cryoprotectants in Vascular Cryopreservation

作　　者：谭丽 王成 陈世玖 TAN Li;WANG Cheng;CHEN Shijiu(Department of Plastic and Hand Surgery,the Fifth Affiliated Hospital of Zunyi Medical University,Zhuhai,Zhuhai 519100,China)

机构地区：[1]遵义医科大学第五附属(珠海)医院整形手外科,广东珠海519100

出　　处：《医学综述》2022年第13期2625-2629,共5页Medical Recapitulate

基　　金：国家自然科学基金(81960824)。

摘　　要：冷冻保护剂(CPA)具有减轻超低温保存损伤的作用,在血管超低温保存中被广泛应用。常见的CPA分为渗透性CPA(如二甲基亚砜、甘油)和非渗透性CPA(如糖类和高分子聚合物)。此外,在超低温保存液中添加间充质干细胞以及一些中药提取物可以促进血管再生,具有保护血管的作用。近年来采用玻璃化超低温保存技术可以有效保护组织及细胞的结构及功能,玻璃化超低温保存技术联合使用无毒性冷冻保护剂或低毒性冷冻保护剂组合可以较好地减少血管冷冻损伤。Cryoprotectants can alleviate cryoinjuries,which are widely used in cryo-preservation of blood vessels.Common cryoprotectants can be categorized into permeable agents(such as dimethylsulphoxide,glycerol)and non-permeable agents(such as glycans and high molecular polymers).Besides,mesenchymal stem cells and some Chinese medicine herbs are added to cryopreservation,which could promote vascular regeneration and protect blood vessels.Recently,cryopreservation by vitrification has been adopted for effective protection of the structure and function of tissues and cells,by combination with nontoxic or low-toxity cryoprotectants to properly reduce vascular cryoinjuries.

关 键 词：血管超低温保存 冷冻保护剂 冷冻损伤 玻璃化保存 

分 类 号：R318.11[医药卫生—生物医学工程]