A hyaluronic acid granular hydrogel nerve guidance conduit promotes regeneration and functional recovery of injured sciatic nerves in rats  

作　　者：Jie Yang Chia-Chen Hsu Ting-Ting Cao Hua Ye Jing Chen Yun-Qing Li 

机构地区：[1]Institute of Medical Research,Northwestern Polytechnical University,Xi’an,Shaanxi Province,China [2]Institute of Biomedical Engineering,Department of Engineering Science,University of Oxford,Oxford,UK [3]The Oxford Suzhou Centre for Advanced Research(OSCAR),Suzhou,Jiangsu Province,China [4]Department of Anatomy,Histology and Embryology&K.K.Leung Brain Research Centre,The Fourth Military Medical University,Xi’an,Shaanxi Province,China [5]Key Laboratory of Brain Science Research and Transformation in Tropical Environment of Hainan Province,Hainan Medical University,Haikou,Hainan Province,China [6]Department of Anatomy,College of Basic Medical Sciences,Dali University,Dali,Yunnan Province,China

出　　处：《Neural Regeneration Research》2023年第3期657-663,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.81620108008(to YQL),31971112(to YQL),82071373(to JC);Innovation Capability Support Program of Shaanxi,No.2021TD-57(to YQL)。

摘　　要：A hyaluronic acid granular hydrogel can promote neuronal and astrocyte colony formation and axonal extension in vitro,suggesting that the hydrogel can simulate an extracellular matrix structure to promote neural regeneration.However,in vivo experiments have not been conducted.In this study,we transplanted a hyaluronic acid granular hydrogel nerve guidance conduit to repair a 10-mm long sciatic nerve gap.The Basso,Beattie,and Bresnahan locomotor rating scale,sciatic nerve compound muscle action potential recording,Fluoro-Gold retrograde tracing,growth related protein 43/S100 immunofluorescence staining,transmission electron microscopy,gastrocnemius muscle dry/wet weight ratio,and Masson’s trichrome staining results showed that the nerve guidance conduit exhibited similar regeneration of sciatic nerve axons and myelin sheath,and recovery of the electrophysiological function and motor function as autologous nerve transplantation.The conduit results were superior to those of a bulk hydrogel or silicone tube transplant.These findings suggest that tissue-engineered nerve conduits containing hyaluronic acid granular hydrogels effectively promote the morphological and functional recovery of the injured sciatic nerve.The nerve conduits have the potential as a material for repairing peripheral nerve defects.

关 键 词：functional recovery granular hydrogel hyaluronic acid myelin sheath nerve conduit nerve regeneration peripheral nerve regeneration sciatic nerve injury tissue engineering transection injury 

分 类 号：R688[医药卫生—骨科学]