睾丸特异性表达基因T279在雄性小鼠精子发生中的作用  

Role of T279 gene in male mouse spermatogenesis

作　　者：王朝亮[1] 孙科[1] 李云龙[1] 毕天祥 乔保平[1] WANG Chaoliang;SUN Ke;LI Yunlong;BI Tianxiang;QIAO Baoping(Department of Urology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院泌尿外科,郑州450052

出　　处：《郑州大学学报（医学版）》2022年第4期552-555,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20190175)。

摘　　要：目的:探索T279基因在雄性小鼠精子发生中的作用。方法:C57BL/6J品系成年雄性T279基因敲除纯合子小鼠(T279^(-/-))12只为实验组,成年雄性野生型小鼠(T279^(+/+))12只为对照组,每只成年雄性小鼠与两只成年野生型雌性小鼠合笼(交配生育实验),1个月后记录雌性小鼠产仔数目,观察两组小鼠泌尿生殖系统的发育情况,HE染色观察睾丸组织形态,采用精子形态学分析技术观察两组小鼠的精子形态。结果:两组小鼠体重和睾丸质量的差异无统计学意义(P>0.05);肾脏、输尿管、膀胱、睾丸、附睾整体形态和大小无明显差异;睾丸精曲小管内均为正常的精子发生状态,各级精子细胞排列有序,可见正常睾丸支持细胞、间质细胞、管周肌样细胞。两组小鼠精子密度和精子活率比较,差异无统计学意义(P>0.05)。实验组小鼠精子畸形率(20.3%)高于对照组(7.4%),差异有统计学意义(P<0.001)。实验组小鼠精子头部畸形。结论:T279基因敲除可导致雄性小鼠精子畸形率明显升高。Aim:To explore the role of T279 gene in male mouse spermatogenesis.Methods:A total of 12 adult male T279 knockout homozygous mice(T279^(-/-))of C57BL/6J strain served as the experimental group,and 12 adult male wild-type mice(T279^(+/+))served as the control group.Each adult male mouse was caged with two adult wild-type female mice(mating fertility experiment).After 1 month,the litter numbers of female mice were recorded,the development of the urogenital system of mice in the two groups was observed,the testis tissue morphology was observed by HE staining,and the sperm morphology of mice in the two groups was observed by sperm morphological analysis technology.Results:There was no significant difference in body weight or testis mass between the two groups(P>0.05);there was no significant difference in the overall shape or size of the kidney,ureter,bladder,testis,and epididymis;the seminiferous tubules in the testis were all in a normal spermatogenesis state.The sperm cells at all levels were arranged orderly,and normal sertoli cells,leydig cells,and peritubular myoid cells could been seen.There was no significant difference in sperm density or sperm motility between the two groups(P>0.05).The sperm abnormality rate of mice in the experimental group(20.3%)was higher than that in the control group(7.4%),and the difference was significant(P<0.001).The mice in the experimental group had sperm head deformities.Conclusion:Knockout of T279 gene can significantly increase the sperm abnormality rate in male mice.

关 键 词：T279 精子发生 精子畸形 小鼠 

分 类 号：R698[医药卫生—泌尿科学]