Mining of candidate genes for grape berry cracking using a genome-wide association study  

作　　者：ZHANG Chuan WU Jiu-yun CUI Li-wen FANG Jing-gui 

机构地区：[1]College of Horticulture,Nanjing Agricultural University,Nanjing 210095,P.R.China [2]Turpan Research Institute of Agricultural Sciences,Xinjiang Academy of Agricultural Sciences,Turpan 838000,P.R.China [3]College of Agro-grassland Sciences,Nanjing Agricultural University,Nanjing 210095,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第8期2291-2304,共14页农业科学学报（英文版）

基　　金：the National Key Research and Development Program of China(2019YFD1000101);the China Agriculture Research System of MOF and MARA(CARS-29-yc-1);Crop Resources Protection Program of Ministry of Agriculture and Rural Affairs of China(2130135–34)。

摘　　要：Fruit cracking is a common phenomenon during the growth and development of horticultural crops that seriously affects fruit yield and quality.However,there are few studies on the mining of candidate genes related to berry cracking.To better understand the genetic basis of grape berry cracking,we conducted a genome-wide association study(GWAS)of grape varieties.Based on the mixed linear model(MLM),we detected five single nucleotide polymorphism(SNP)loci associated with berry-cracking index and two SNP loci associated with berry-cracking type in two years.These loci were mainly distributed on four chromosomes,namely 1,2,3,and 18,and were associated with ten unique candidate berry-cracking genes.The gene expression patterns indicated that the candidate genes in the susceptible berrycracking variety were more abundant than in the resistant berry-cracking variety.Grape berry-cracking is a complex trait controlled by multiple genes,mainly including genes involved in polygalacturonase,copper transporter,and receptor-like proteins.The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking,so the present study helps to further elucidate the genetic mechanism of berry cracking.

关 键 词：berry-cracking index berry-cracking type GWAS MLM SNP 

分 类 号：S663.1[农业科学—果树学]