2型糖尿病患者环状RNA_0005414的表达水平与胰岛细胞功能的相关性研究  

Relationship between the circular RNA_0005414 expression level and the islet cell function in patients with type 2 diabetes mellitus

作　　者：朱余蓉 常向云[2] 余学锋[1] 巴涛 孙媛 张剑萍 Zhu Yurong;Chang Xiangyun;Yu Xuefeng;Ba Tao;Sun Yuan;Zhang Jianping(Department of Endocrinology and Metabolism,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,China;Department of Endocrinology and Metabolism,the First Affiliated Hospital,Shihezi University School of Medicine,Shihezi 832008,China;Second Department of Internal Medicine,the 51st Regiment Hospital of the Third Division of Xinjiang,Tumushuk 843806,China)

机构地区：[1]华中科技大学同济医学院附属同济医院内分泌代谢科,武汉430000 [2]石河子大学医学院第一附属医院内分泌代谢科,石河子832008 [3]新疆第三师51团医院内二科,图木舒克843806

出　　处：《中华内分泌代谢杂志》2022年第5期369-374,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金(81860149)。

摘　　要：目的探讨2型糖尿病患者环状RNA_0005414的表达水平与胰岛细胞功能的关系。方法共纳入维吾尔族2型糖尿病患者110例、维吾尔族糖耐量正常者(对照组)106名以及汉族2型糖尿病患者64例、汉族糖耐量正常者(对照组)63名。所有研究对象进行口服葡萄糖耐量试验并检测生化指标,以稳态模型评估的胰岛素抵抗指数(HOMA-IR)、稳态模型评估的胰岛β细胞功能指数(HOMA-β)作为胰岛细胞功能评价指标。分别在维吾尔族2型糖尿病患者、糖耐量正常者各选取匹配的5例研究对象的外周血单核细胞通过RNA测序筛选差异表达的环状RNA,检测上调显著的一条环状RNA_0005414表达水平,分析环状RNA_0005414的表达水平与胰岛细胞功能的关系。结果维吾尔族2型糖尿病患者中存在环状RNA差异表达谱;维吾尔族2型糖尿病组环状RNA_0005414表达水平高于对照组(P<0.01),汉族2型糖尿病组环状RNA_0005414表达水平亦高于对照组(P<0.01),维吾尔族2型糖尿病组环状RNA_0005414表达水平较汉族2型糖尿病组有升高趋势,但差异无显著性(P>0.05)。在维吾尔族和汉族各组中,Spearman相关分析显示,环状RNA_0005414与空腹血糖、糖负荷后2 h血糖、HbA_(1C)、总胆固醇、空腹胰岛素、HOMA-IR呈正相关(P<0.05),与HOMA-β呈负相关(P<0.01),偏相关分析显示,环状RNA_0005414与空腹血糖、HbA_(1C)、HOMA-IR呈正相关(P<0.05)。多重线性回归分析显示,仅在维吾尔族2型糖尿病患者中,环状RNA_0005414是HOMA-β的影响因素。结论维吾尔族2型糖尿病患者环状RNA_0005414表达水平与胰岛细胞功能密切相关,环状RNA_0005414表达水平上调可能参与了维吾尔族2型糖尿病的发生发展。Objective To investigate the relationship between the expression level of circular RNA_0005414 and islet cell function in type 2 diabetes patients.Methods A total of 110 patients with type 2 diabetes and 106 cases of normal glucose tolerance(control group)in Uygur populations,64 cases of type 2 diabetes mellitus and 63 cases of normal glucose tolerance(control group)in Han populations were enrolled in the study.All subjects underwent oral glucose tolerance test chemistry panel.Homeostasis model assessment for insulin resistance(HOMA-IR)andβcell function(HOMA-β)were evaluated by homeostasis model as islet cell function indexes.The differentially expressed circular RNAs were screened using RNA sequencing from the peripheral blood monocytes of 5 Uygur patients with type 2 diabetes mellitus and matched controls.The expression level of a significantly up-regulated circular RNA_0005414 was detected and verified,and the relationship between the expression level of circular RNA_0005414 and islet cell function was analyzed.Results Differential expression profiles of circular RNAs were found in Uygur type 2 diabetic patients.The expression level of circular RNA_0005414 in Uygur type 2 diabetic group was higher than that in Uygur control group(P<0.01),the expression level of circular RNA_0005414 in Han type 2 diabetic group was higher than that in Han control group(P<0.01),the expression level of circular RNA_0005414 in Uygur type 2 diabetes group was higher than that in Han type 2 diabetes group,but the difference was not statistically significant(P>0.05).In the Uyghur and Han groups,Spearman correlation analysis showed that the expression level of circular RNA_0005414 was positively correlated with fasting blood glucose,2 h plasma glucose after glucose loading,HbA_(1C),total cholesterol,fasting insulin,HOMA-IR(P<0.05)and negatively correlated with HOMA-β(P<0.01).Partial correlation analysis showed that circular RNA_0005414 expression level was positively correlated with fasting blood glucose,HbA_(1C),and HOMA-IR(P<0.01

关 键 词：维吾尔族 汉族 糖尿病 2型 环状RNA 胰岛细胞功能 

分 类 号：R587.1[医药卫生—内分泌]