基于环烯醚萜苷类成分评价不同种质地黄的质量  

Evaluation of the quality of different germplasms of Rehmannia glutinosa based on iridoid glycosides

作　　者：耿晓桐 谢彩侠[2] 张楠 李金平 陈琼[1] GENG Xiaotong;XIE Caixia;ZHANG Nan;LI Jinping;CHEN Qiong(School of Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Henan Xinyang 464000,China;College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450000,China;Admissions Employment Office,Xinyang Normal University,Henan Xinyang 464000,China;Henan Xibanxia Pharmaceutical Company Limited,Henan Xinyang 464000,China)

机构地区：[1]信阳农林学院制药工程学院,河南信阳464000 [2]河南中医药大学药学院,郑州450000 [3]信阳师范学院招生就业处,河南信阳464000 [4]河南息半夏药业有限公司,河南信阳464000

出　　处：《中国药房》2022年第15期1833-1837,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.82073952);2020年度河南省创新示范专项(No.201111310900)。

摘　　要：目的基于环烯醚萜类成分评价不同种质地黄的质量。方法以6种种质(85-5、金九、BX、BJ-1、山东、QH-1)共18批地黄药材为研究对象,采用紫外分光光度法和高效液相色谱法测定地黄中总环烯醚萜苷、梓醇、地黄苷D、地黄苷A、益母草苷的含量;将上述含量测定结果经归一化处理后,采用聚类分析、因子综合分析及偏最小二乘判别分析(PLS-DA)等多元统计学方法评价不同种质地黄的质量。结果6种种质地黄中总环烯醚萜苷含量最高的是85-5地黄,梓醇含量最高的是BX地黄,地黄苷D、地黄苷A含量最高的是金九地黄,益母草苷含量最高的是BX地黄。进一步经聚类分析发现,金九地黄聚为一类,BX地黄聚为一类,山东、BJ-1地黄聚为一类,QH-1、85-5地黄聚为一类。经因子综合分析发现,不同种质地黄的质量存在差异,BX、山东、85-5、BJ-1、QH-1、金九地黄的综合得分分别为2.2839、1.6891、1.6648、1.5033、1.4690、1.2146。经PLS-DA发现,总环烯醚萜苷、梓醇、益母草苷的变量重要性投影值均大于1。结论不同种质地黄的质量差异可能是由总环烯醚萜苷、梓醇、益母草苷这3种成分造成的。OBJECTIVE To evaluate the quality of different germplasms of Rehmannia glutinosa based on iridoid glycosides.METHODS The contents of total iridoid glycosides,catalpol,rehmaionoside D,rehmaionoside A,and leonuride in 18 batches of R.glutinosa from 6 germplasms(85-5,JinJiu,BX,BJ-1,Shandong,QH-1)were determined by ultraviolet spectrophotometry and high performance liquid chromatography.After normalization of the above content determination results,the quality of different germplasm of R.glutinosa were evaluated by multiple statistical methods such as cluster analysis,factor comprehensive analysis and partial least squares discriminant analysis(PLS-DA).RESULTS Among 6 germplasms of R.glutinosa,the content of total iridoid glycosides in R.glutinosa 85-5 was the highest,and the content of catalpol in R.glutinosa BX was the highest;the contents of rehmannioside D and rehmannioside A in R.glutinosa JinJiu were the highest,and the content of leonuride in R.glutinosa BX was the highest.Cluster analysis showed that R.glutinosa JinJiu were clustered into one category,R.glutinosa BX clustered into one category,R.glutinosa Shandong and R.glutinosa BJ-1 were clustered into one category,and R.glutinosa QH-1 and 85-5 were clustered into one category.Through factor comprehensive analysis,there were differences in the quality of different germplasms of R.glutinosa.The comprehensive score of R.glutinosa BX,Shandong,85-5,BJ-1,QH-1,JinJiu were 2.2839,1.6891,1.6648,1.5033,1.4690,1.2146,respectively.PLS-DA showed that variable importance projection value of total iridoid glycosides,catalpol and leonuride were all higher than 1.CONCLUSIONS The quality difference of R.glutinosa from different germplasms may be caused by total iridoid glycosides,catalpol and leonuride.

关 键 词：地黄 种质 环烯醚萜苷 多元统计学 质量评价 

分 类 号：R917[医药卫生—药物分析学] R282[医药卫生—药学]