柚皮苷转化生成红花素及异红花素的研究  

Biotransformation of naringin to carthamidin and isocarthamidin by Aspergillus japonicus

作　　者：李业英[1] 马瑛[1] 崔晓兰[1] 尹力新 穆云龙[1] 单越琦[1] 路新华[1] 沈文斌 Li Ye-ying;Ma Ying;Cui Xiao-lan;Yin Li-xin;Mu Yun-long;Shan Yue-qi;Lu Xin-hua;Shen Wen-bin(National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Engineering&Technology Research Center,New Drug Research&Development Company of NCPC,Shijiazhuang 052165;North China Pharmaceutical Co.,Ltd,Shijiazhuang 050015)

机构地区：[1]微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,华北制药集团新药研究开发有限责任公司,石家庄052165 [2]华北制药股份有限公司,石家庄050015

出　　处：《中国抗生素杂志》2022年第6期556-560,共5页Chinese Journal of Antibiotics

基　　金：河北省工业微生物代谢工程技术研究中心运行绩效后补助经费(No.199676154H)。

摘　　要：目的 利用一株日本曲霉,以柚皮苷为底物,转化生成红花素和异红花素并提高产物的发酵单位。方法 对发酵培养基中氮源种类进行筛选并确定最适的添加浓度,考察微量元素对发酵的影响并确定最适添加浓度,降低溶解底物的乙醇浓度并采用二次添加底物的方式等。结果 红花素发酵单位由66μg/mL提高至389μg/mL,异红花素发酵单位由263μg/mL提高至946μg/mL。热豆粉是最佳氮源,添加浓度为0.3%;添加硫酸亚铁和氯化锰能够分别促进红花素和异红花素的生成,其最适添加浓度分别为0.02%和0.01%;溶解底物的乙醇浓度由50%降低至26.6%,降低了乙醇对转化的不利影响;底物添加次数由1次变为2次,有利于发酵单位的提高。结论 首次发现日本曲霉可直接转化柚皮苷生成红花素和异红花素,且柚皮苷生物转化为红花素和异红花素的发酵单位较高。Objective To optimize the fermentation medium and conditions of biotransformation by Aspergillus japonicus, which could biotransform naringin to carthamidin and isocarthamidin, so as to improve the fermentation titer of carthamidin and isocarthamidin. Methods The effects of nitrogen sources and the content of optimum nitrogen source and trace elements on biotransformation were investigated. The conditions for biotransformation such as the shaker speed and substrate addition methods were optimized. Results Soybean meal was the optimum organic nitrogen source and the optimum content was 0.3%. FeSOand MnClcould improve the production of carthamidin and isocarthamidin respectively. The content of alcohol in substrate solution was reduced from 50% to 26.6%. Finally the titer of carthamidin and isocarthamidin reached 389 μg/mL and 946 μg/mL. Conclusions Naringin could be directly and efficiently transformed to carthamidin and isocarthamidin by Aspergillus japonicus.

关 键 词：柚皮苷 红花素 异红花素 生物转化 日本曲霉 

分 类 号：Q978.1[生物学]