IL-17、IL-35联合补体C3、C4在CHB患者外周血中的表达及与肝纤维化程度的关系研究  

Expression of IL-17 and IL-35 combined with complement C3 and C4 in peripheral blood of patients with CHB and its relationship with liver fibrosis

作　　者：王海东 李宁侠 李建华[1] 唐小璐[2] 李江 董恒利[1] WANG Haidong;LI Ningxia;LI Jianhua;TANG Xiaolu;LI Jiang;DONG Hengli(Department of Clinical Laboratory,the Second Affiliated Hospital of Xi'an Medical College,Xi'an,Shaanxi 710038,China;Department of Neurosurgery,the Second Affiliated Hospital of Xi'an Medical College,Xi'an,Shaanxi 710038,China;Department of Blood Transfusion,Xianyang Hospital,Yan'an University,Xianyang,Shaanxi 712099,China)

机构地区：[1]西安医学院第二附属医院检验科,陕西西安710038 [2]西安医学院第二附属医院神经外科,陕西西安710038 [3]延安大学咸阳医院输血科,陕西咸阳712099

出　　处：《检验医学与临床》2022年第15期2097-2100,共4页Laboratory Medicine and Clinic

摘　　要：目的分析白细胞介素(IL)-17、IL-35、补体C3、C4在慢性乙型肝炎(CHB)患者外周血中的表达及与肝纤维化程度的关系。方法选取西安医学院第二附属医院2019年1月至2020年2月收治的110例CHB患者作为观察组,100例体检健康者作为对照组;收集血清检测血中IL-17、IL-35、补体C3和补体C4水平;采用Logistic回归模型分析IL-17、IL-35、补体C3、补体C4水平与患者肝纤维化程度的关系。结果观察组血中IL-17、IL-35水平明显高于对照组(P<0.05),观察组血中补体C3及补体C4水平明显低于对照组(P<0.05);不同肝纤维化程度患者血中IL-17、IL-35、补体C3、补体C4水平比较,差异均有统计学意义(P<0.05),其中F0期患者血中IL-17、IL-35水平低于F1~F2期和F3~F4期患者(P<0.05),F1~F2期患者血中IL-17、IL-35水平低于F3~F4期患者(P<0.05),F0期患者血中补体C3、补体C4水平高于F1~F2期和F3~F4期患者(P<0.05),F1~F2期患者血中补体C3、补体C4水平高于F3~F4期患者(P<0.05);血中高IL-17及IL-35水平是CHB患者肝纤维化的独立性危险因素(P<0.05),血中高补体C3及补体C4水平是CHB患者纤维化的独立性保护因素(P<0.05)。结论CHB患者外周血中IL-17、IL-35水平明显升高,补体C3、补体C4水平明显降低,患者血中IL-17、IL-35、补体C3及C4补体是CHB患者肝纤维化的独立性影响因素。Objective To analyze the expression of interleukin(IL)-17,IL-35,complement C3 and C4 in peripheral blood of patients with chronic hepatitis B(CHB)and the relationship with the degree of liver fibrosis.Methods Totally 110 patients with CHB admitted to the Second Affiliated Hospital of Xi'an Medical College from January 2019 to February 2020 were enrolled as the observation group,and 100 healthy subjects were used as the control group.Serum samples were collected for serum IL-17,IL-35,complement C3 and complement C4 levels.Logistic regression model was used to analyze the relationship between IL-17,IL-35,complement C3,and complement C4 levels and liver fibrosis.Results The levels of IL-17 and IL-35 in the observation group were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).The levels of complement C3 and complement C4 in the observation group were significantly lower than those in the control group,and the differences were statistically significant(P<0.05).There were significant differences in the levels of IL-17,IL-35,complement C3 and complement C4 in patients with different degrees of fibrosis(P<0.05).Among them,the levels of IL 17 and IL 35 in patients with F0 stage were lower than those in patients with F1-F2 stage and F3-F4 stage(P<0.05),the levels of IL-17 and IL-35 in patients with F1-F2 stage were lower than those in patients with F3-F4 stage(P<0.05),the levels of complement C3 and C4 in patients with F0 stage were higher than those in patients with F1-F2 stage and F3-F4 stage(P<0.05),and the levels of complement C3 and C4 in patients with F1-F2 stage were higher than those in patients with F3-F4 stage(P<0.05).IL-17 and IL-35 in blood were independent risk factors for liver fibrosis in patients with CHB(P<0.05),and complement C3 and C4 in blood were independent protective factors for fibrosis in patients with CHB(P<0.05).Conclusion In peripheral blood of patients with CHB,IL-17 and IL-35 levels were significantly increased,and complement C3

关 键 词：白细胞介素-17 白细胞介素-35 补体C3 补体C4 慢性乙型肝炎 肝纤维化 

分 类 号：R446.1[医药卫生—诊断学]