lncRNAs H19在糖尿病骨关节炎软骨下骨中的表达及其意义  

Expression of lncRNAs H19 and its significance in subchondral bone of diabetic mellitus osteoarthritis

作　　者：薛伟[1] 康少英[1] 郭洪生[1] 王培霞[1] 王振兴[1] 詹金华[1] 张英民[1] 王华军[2] XUE Wei;KANG Shaoying;GUO Hongsheng;WANG Peixia;WANG Zhenxing;ZHAN Jinhua;ZHANG Yingmin;WANG Huajun(The Third Department of Orthopedics,Handan Central Hospital,Hebei Province,Handan 056001,China;Bone Joint and Sports Medical Center,the First Clinical College of Jinan Universitythe First Affiliated Hospital of Jinan University,Guangdong Province,Guangzhou 510630,China)

机构地区：[1]河北省邯郸市中心医院骨三科,河北邯郸056001 [2]暨南大学第一临床医学院暨南大学附属第一医院骨关节与运动医学中心,广东广州510630

出　　处：《中国医药导报》2022年第20期25-29,共5页China Medical Herald

基　　金：广东省医学科学技术研究基金项目(A2019100)。

摘　　要：目的探究lncRNAs H19在糖尿病骨关节炎(OA)软骨下骨中的表达及其意义。方法选取2017年1月至2019年6月于河北省邯郸市中心医院就诊的糖尿病OA患者(DM-OA组)与非糖尿病OA患者(OA组)各3例,以及同期体检的对照组3名。对比H19在各组软骨下骨成骨细胞中的表达,构建H19过表达质粒,合成miR-141、miR-22 mimic类似物;检测β-catenin蛋白的表达量;检测成骨细胞分化功能。结果DM-OA组的H19表达水平高于OA组及对照组,miR-141、miR-22表达水平低于OA组及对照组(P<0.05);OA组的H19表达水平高于对照组,miR-141、miR-22表达水平低于对照组(P<0.05)。过表达H19 WT和miR-141、miR-22片段后,荧光素酶报道的荧光信号明显减弱(P<0.01)。过miR-141 mimic、miR-22 mimic相似物对突变质粒的荧光信号不产生影响(P>0.05)。DM-OA组的β-catenin mRNA、Runx2 mRNA表达水平高于OA组及对照组(P<0.05);OA组的β-catenin mRNA、Runx2 mRNA表达水平高于对照组(P<0.05)。DM-OA组的β-catenin蛋白、Runx2蛋白表达水平高于OA组及对照组(P<0.05);OA组的β-catenin蛋白、Runx2蛋白表达水平高于对照组(P<0.05)。结论H19在糖尿病OA软骨下骨中过表达,H19可通过调控miR-141、miR-22激活Wnt/β-catenin信号通路促进软骨下骨增生硬化。Objective To investigate the expression of lncRNAs H19 in the subchondral bone of diabetic mellitus osteoarthritis(OA)and its significance.Methods Three patients with diabetes mellitus OA(DM-OA group),three patients with non diabetes OA(OA group)and,meanwhile,three physical examinees of the control group were selected.The expression of H19 in subchondral bone of each group was compared,H19 overexpression plasmid was constructed,and miR-141 and miR-22 mimic analogues were synthesized.The expression of β-catenin protein was detected.The differentiation function of osteoblasts was detected.Results The expression level of H19 in DM-OA group was higher than that in OA group and control group,and the expression levels of miR-141 and miR-22 in DM-OA group were lower than those in OA group and control group(P<0.05).The expression level of H19 in OA group was higher than that in control group,and the expression levels of miR-141 and miR-22 in OA group were lower than those in control group(P<0.05).After overexpression of H19 WT,miR-141,and miR-22 fragments,the fluorescence signal reported by luciferase decreased significantly(P<0.01).The miR-141 mimic and miR-22 mimic analogues had no effect on the fluorescence signal of mutant plasmids,and the difference was not statistically significant(P>0.05).The expression levels of β-catenin mRNA and Runx2 mRNA in DM-OA group were higher than those in OA group and control group(P<0.05).The expression levels of β-catenin mRNA and Runx2 mRNA in OA group were higher than those in control group(P<0.05).The expression levels of β-catenin protein and Runx2 protein in DM-OA group were higher than those in OA group and control group(P<0.05).The expression levels of β-catenin protein and Runx2 protein in OA group were higher than those in control group(P<0.05).Conclusion H19 is overexpressed in the subchondral bone of diabetes OA.H19 can activate Wnt/β-catenin signaling pathway by regulating miR-141 and miR-22 promotes subchondral bone hyperplasia and sclerosis.

关 键 词：骨关节炎 软骨下骨 成骨细胞 H19 

分 类 号：R684.3[医药卫生—骨科学]