兔出血症病毒2型感染的病例诊断  

Diagnosis of a Rabbit Hemorrhagic Virus Type 2 Infection Epidemic

作　　者：常赵阳 刘玉梅[1] 刘潍淇 王佳宁 位兰兰 张自强[1] CHANG Zhaoyang;LIU Yumei;LIU Weiqi;WANG Jianing;WEI Lan;ZHANG Ziqiang(College of Animal Science and Technology,Henan Universily of Science and Technology,Luoyang 471000)

机构地区：[1]河南科技大学动物科技学院,洛阳471000

出　　处：《中国养兔》2022年第3期4-9,共6页Chinese Journal of Rabbit Farming

基　　金：河南省重点研发与推广专项(192102110077,202102110093)。

摘　　要：为了解河南疑似兔出血症病毒2型(RHDV)的感染情况,并对RHDV的致病性进行初步分析。研究采集病死兔的肝组织,利用微量血凝试验、RT-PCR扩增及测序、VP60基因系统进化树分析和动物回归试验进行病原鉴定。微量血凝试验结果显示,组织样本悬液能够凝集人“O”型血红细胞;RT-PCR扩增、测序及序列分析结果显示,检测到RHDV特异性条带,片段大小为829 bp;系统进化树分析结果发现,分离的病毒与我国四川发现的首例RHDV毒株SC2020/04的VP60基因相似性高达98.2%;临床病例的剖检显示病死兔胸腺、气管、肺、肝、脾、肾等实质性器官出血较为严重;动物回归试验发现攻毒组家兔病死率为100%,平均死亡时间为65.8 h,RT-PCR扩展均检测到RHDV特异性条带。该研究是首次在河南兔场检测到RHDV,为RHDV的防控提供科学参考。In order to understand the infection situation of suspected rabbit hemorrhagic virus type 2 (RHDV) in Henan province,and a preliminary analysis of the pathogenicity of RHDVwas conducted.This study collected the liver tissues of dead rabbits and used microblood coagulation assays,RT-PCR amplification and sequenc-ing,VP60 gene phylogenetic tree analysis and animal regression test to identified the pathogen.The results of the microblood coagulation assay showed that the tissue sample suspension were able to produce a lect reaction with the human type"O"red blood cells;RT-PCR showed the detection of RHDV-specific bands,the fragment size was 829 bp;Results of the phylogenetic tree analysis showed that the VP60 gene similarity was as high as98.2%between the isolated virus and the first RHDVstrain SC2020/04 found in Sichuan;The autopsy of clini-cal cases showed that the bleeding of thymus,trachea,lung,liver,spleen,kidney and other substantial organs was more serious;Animal regression test showed that the mortality rate of rabbits was 100%and the average death time was 65.8h after challenge test,and the specific bands of RHDVwere detected by RT-PCR amplifi-cation.In conclusion,the RHDVvirus was detected for the first time in the rabbit field in Henan Province which provided a scientific reference for the scientific and reasonable prevention and control of RHDV.

关 键 词：兔出血症病毒1型 兔出血症病毒2型 VP60基因 RT-PCR 致病力 

分 类 号：S829.1[农业科学—畜牧学]