阿托伐他汀通过NFATc1信号通路促进大鼠骨质疏松性骨折愈合  

Atorvastatin promoting osteoporotic fracture healing in rats through NFATc1 signaling pathway

作　　者：王琮仁[1] 孔长庚[1] 郭祥[1] 金旭红[1] 吴多庆[1] 范忠诚[1] WANG Congren;KONG Changgeng;GUO Xiang(Orthopedic Medical Center,Haikou Hospital Affiliated to Xiangya School of Medicine,Central South University,Hainan 572000,China)

机构地区：[1]中南大学湘雅医学院附属海口医院骨科医学中心,海南572000

出　　处：《临床外科杂志》2022年第6期583-585,共3页Journal of Clinical Surgery

摘　　要：目的研究阿托伐他汀对大鼠骨质疏松性骨折(OPF)愈合的影响及相关机制。方法40只大鼠建立OPF模型,随机分为模型组,实验A、B、C组,各10只。实验A、B、C组灌喂阿托伐他汀10、20、40 mg/kg,模型组灌喂等体积生理盐水。X光片观察骨折愈合情况;生物力学测试检测骨痂生物力学性能;免疫印迹法检测骨痂组织活化T细胞核因子1(NFATc1)、破骨细胞关联受体(OSCAR)、骨钙素(OCN)、I型胶原(COL-I)蛋白表达量。结果与模型组比较,实验A组X光片评分升高,最大载荷、刚度增加,NFATc1、OSCAR蛋白表达量降低,OCN、COL-I蛋白表达量升高,差异有统计学意义(P<0.05),且表现为剂量依赖性。结论阿托伐他汀可促进大鼠OPF愈合,增强生物力学性能,其作用机制与抑制NFATc1信号通路有关。Objective To observe the effect of atorvastatin on the healing of osteoporotic fractures(OPF)in rats and related mechanisms.Methods Forty rats were established OPF models.They were randomly divided into model group,experimental-A group,experimental-B group,and experimental-C group,each with 10 rats.Experimental A,B,and C groups were given atorvastatin 10,20,and 40 mg·kgrespectively,and the model group was given an equal volume of normal saline.The fracture healing in rats were observed by X-ray film.The biomechanical properties of callus were detected by biomechanical test.The protein expression of activated T cell nuclear factor 1(NFATc1)and osteoclast-associated receptor(OSCAR),osteocalcin(OCN),collagen Type I(COL-I)in the callus tissue were detect by Western blotting.Results Compared with the model group,the X-ray scores of experimental-A group was increased,the maximum load and stiffness was increased,the protein expressions of NFATc1 and OSCAR were decreased,and the protein expressions of OCN and COL-I were increased(P<0.05),and ot was dose-dependent.Conclusion Atorvastatin can promote the healing of OPF in rats and enhance the biomechanical properties.It is speculated that its mechanism of action is related to the inhibition of the NFATc1 signaling pathway.

关 键 词：阿托伐他汀 骨质疏松性骨折 活化T细胞核因子 

分 类 号：R965[医药卫生—药理学]