虫草素对肺癌细胞生长及迁移的影响  

Effects of cordycepin on the growth and migration of lung cancer cells

作　　者：宋羚 田迪 黄蓉 刘新会 罗家兴 马啸[1,3] SONG Ling;TIAN Di;HUANG Rong;LIU Xinhui;LUO Jiaxing;MA Xiao(College of Tea Science,Yunnan Agricultural University,Kunming 650201,Yunnan,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Yunnan Highland Special Agricultural Industry Research Institute,Kunming 650201,Yunnan,China)

机构地区：[1]云南农业大学茶学院,云南昆明650201 [2]云南农业大学食品科学技术学院,云南昆明650201 [3]云南省高原特色农业产业研究院,云南昆明650201

出　　处：《菌物学报》2022年第7期1088-1098,共11页Mycosystema

基　　金：云南省高层次人才培养支持计划青年拔尖人才专项(YNWR-QNBJ-2018-173);云南省重大科技专项:绿色食品国际合作研究中心项目(2019ZG009);云南省科技项目(YCJ 2020-323);云南科技成果转移支持项目(K2120200001-2101)。

摘　　要：探讨虫草素对非小细胞肺癌细胞株H1781细胞凋亡及迁移的影响及作用机制。培养H1781细胞并分组,对照组用不含药物的培养基处理,虫草素组用含有10、20、30和40μmol/L虫草素处理,处理24h后测定细胞活力,通过显微镜观察细胞形态学,HE染色观察虫草素对细胞整体的影响,细胞免疫荧光技术检测细胞中MMP-9和DAPI核染色情况观察细胞凋亡,Western blotting检测凋亡等相关蛋白表达。与对照组相比,虫草素处理24 h后,H1781细胞系活力显著降低;细胞数量明显减少;HE染色观察发现随着虫草素浓度增加,细胞数量及细胞集团明显变少,免疫荧光技术检测发现药物处理后细胞凋亡明显促进;划痕实验发现虫草素明显降低细胞迁移能力;Western blotting实验中Bax、cleaved caspase-3蛋白表达明显上调,MMP-9、Bcl-2蛋白表达明显下调。虫草素对肺癌H1781细胞迁移有抑制作用、对凋亡有促进作用,推测其作用机制为上调促凋亡蛋白表达、下调抗凋亡蛋白表达。The effect of cordycepin on apoptosis and migration of human bronchoalveolar carcinoma cell line H1781 cells and its mechanism of action were investigated.The H1781 cells were cultured and grouped,and the blank control group was treated with drug-free medium,while the cordycepin treatment group was treated with cordycepin of 10,20,30 and 40μmol/L.Cell viability was measured after 24 h of treatment;cell morphology was observed by microscopy;the effect of cordycepin on the whole cells was observed by HE staining,and apoptosis was detected by MMP-9 and DAPI nuclear staining using cell immunofluorescence method.The apoptosisrelated protein expression was detected by Western blotting.Compared with the blank control,the viability of H1781 cell line was significantly reduced after 24 h of cordycepin treatment;the number of cells was significantly reduced.HE staining revealed that the number of cells and cell groups became significantly less with the increase of cordycepin concentration,and the immunofluorescence detection revealed that apoptosis was significantly promoted after drug treatment;the scratching experiment revealed that cordycepin significantly reduced cell migration ability;Western blotting assay showed the expression of Bax and cleaved caspase-3 proteins were significantly up-regulated and the expression of MMP-9 and Bcl-2 proteins were significantly down-regulated.The results come to the conclusion that cordycepin can inhibit the migration of lung cancer H1781 cells and promote apoptosis,and these probably result from up-regulation of pro-apoptotic protein expression and down-regulation of anti-apoptotic protein expression.

关 键 词：虫草素 非小细胞肺癌 天然产物 凋亡 迁移 机制研究 

分 类 号：R285[医药卫生—中药学]