基于粪便组学的野生哺乳动物肠道微生态研究进展  

Advances on Intestinal Microecology Based upon Omics Analyses of Feces Samples in Wild Mammals

作　　者：甄军爱 杨亚昕 朱轶强 任义军 陈玉清[1] ZHEN Jun ai;YANG Yaxin;ZHU Yiqiang;REN Yijun;CHEN Yuqing(Life Sciences College,Nanjing Normal University,Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology,Nanjing,210023,China;Jiangsu Dafeng Pere David Deer National Nature Reserve Management Office,Dafeng,224136,China)

机构地区：[1]南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,南京210023 [2]江苏省大丰麋鹿国家级自然保护区管理处,大丰224136

出　　处：《野生动物学报》2022年第3期836-844,共9页CHINESE JOURNAL OF WILDLIFE

基　　金：江苏省林业科技创新与推广项目(Grant NO.LYKJ[2019]49)。

摘　　要：野生哺乳动物肠道微生态由肠道正常菌群及其所生活的环境共同构成,与动物个体生理和健康密切相关。粪便样品对野生动物肠道微生态的研究具有特殊价值,基于粪便组学将可能从多个角度对野生哺乳动物肠道微生态在外界环境、饮食和疾病等方面提供重要信息。本文主要综述了基于粪便的高通量组学技术及其在野生哺乳动物研究中的应用,重点关注野生与圈养、气候与地域变化,以及疾病状态的肠道微生物组学和代谢组学的研究应用,以期推动生物技术在我国野生哺乳动物研究中的发展,为野生动物保护提供新的研究手段和研究方向。The‘intestinal microecology’of wild mammals consists of intestinal microbiota,intestinal epithelial cells,and mucosal immune system that together form the intestinal mucosal barrier,and plays an important role in the regulation of the physiology and health of individuals.Because of the special values of fecal samples for the study of intestinal microecology in wild mammals,omics analysis based upon feces sample may provide important information for interpreting the intestinal microecology of wild mammalian respect to the environment,diet,disease and so on.In this review,we introduced several high-throughput omics technologies using feces samples.We focused on their application on different research area in both wild and captive mammals,as well as on varying climatic,geographical,and healthy conditions.We hope to promote the application of biotechnology in the research of wild mammals in China,and to provide a new research approach and direction for wild animal protection.

关 键 词：野生哺乳动物 粪便 代谢组学 微生物组学 蛋白质组学 

分 类 号：Q938[生物学—微生物学]