玉米3βHSD/D基因家族的鉴定和功能分析  

Identification and Functional Characterization of Maize 3βHSD/D Sterol Biosynthesis Enzymes

作　　者：王萌 门淑珍[1] Wang Meng;Men Shuzhen(College of Life Sciences,Nankai University,Tianjin 300071 China)

机构地区：[1]南开大学生命科学学院植物生物学和生态学系,天津300071

出　　处：《南开大学学报（自然科学版）》2022年第3期61-66,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(31570247,31870230)。

摘　　要：4α-羧基固醇C3-脱氢酶/C4-脱羧酶(4α-carboxysterol-C3-dehydrogenase/C4-decarboxylase,3βHSD/D)在植物固醇合成途径中起到氧化和异构固醇前体的作用.利用玉米基因组数据库,通过生物信息学手段鉴定玉米3βHSD/D家族,阐明该家族成员的基本结构情况,系统发育进化关系.通过酵母回补实验验证了玉米Zm3βHSD/D1和Zm3βHSD/D3的功能,并对两者进行了蛋白拓扑结构预测.RT-PCR结果显示,Zm3βHSD/D1在玉米多个组织器官中都有表达,Zm3βHSD/D3在子房和胚芽鞘中表达较强.4α-carboxysterol-C3-dehydrogenase/C-4-decarboxylase(3βHSD/D)is an essential enzyme in sterol biosynthesis pathway.Based on maize database and bioinformatics,maize 3βHSD/D family genes with their gene structures and phylogenetic relationships were obtained.Functional validation of Zm3βHSD/D1 and Zm3βHSD/D3 was carried out by yeast erg26 mutant complementation.RT-PCR analysis indicated that Zm3βHSD/D1 was expressed in most analyzed organs,whereas Zm3βHSD/D3 was only expressed in coleoptile,ovary and root.

关 键 词：植物固醇 生物信息学 玉米 3βHSD/D 

分 类 号：Q943[生物学—植物学]